Abstract
A protocol for production of bovine embryos from oocytes collected from ovaries obtained from an abattoir is described. The protocol includes methods for in vitro maturation of oocytes, capacitation of sperm, fertilization, and development of the resultant embryos to the blastocyst stage. The protocol can be easily modified to use oocytes collected by ultrasound-guided follicular aspiration.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Brackett BG, Bousquet D, Boice ML, Donawick WJ, Evans JF, Dressel MA (1982) Normal development following in vitro fertilization in the cow. Biol Reprod 27:147–158
Block J, Bonilla L, Hansen PJ (2009) Effect of addition of hyaluronan to embryo culture medium on survival of bovine embryos in vitro following vitrification and establishment of pregnancy after transfer to recipients. Theriogenology 71:1063–1071
Bouquet A, Juga J (2013) Integrating genomic selection into dairy cattle breeding programmes: a review. Animal 7:705–713
Baruselli PS, Ferreira RM, Sales JN, Gimenes LU, Sá Filho MF, Martins CM, Rodrigues CA, Bó GA (2011) Timed embryo transfer programs for management of donor and recipient cattle. Theriogenology 76:1583–1593
Hansen PJ (2017) Physiological approaches to improving fertility during heat stress. In: Beede DK (ed) Large dairy herd management, 3rd edn. American Dairy Science Assn, Champaign, IL, pp 7–42
Hansen PJ, Block J, Loureiro B, Bonilla L, Hendricks KE (2010) Effects of gamete source and culture conditions on the competence of in vitro-produced embryos for post-transfer survival in cattle. Reprod Fertil Dev 22:59–66
Galli C, Duchi R, Colleoni S, Lagutina I, Lazzari G (2014) Ovum pick up, intracytoplasmic sperm injection and somatic cell nuclear transfer in cattle, buffalo and horses: from the research laboratory to clinical practice. Theriogenology 81:138–151
Leivas FG, Brum DS, Fialho SS, Saliba WP, Alvim MT, Bernardi ML, Rubin MI, Silva CA (2011) Fetal calf serum enhances in vitro production of Bos taurus indicus embryos. Theriogenology 75:429–433
Pinyopummintr T, Bavister BD (1994) Development of bovine embryos in a cell-free culture medium: Effects of type of serum, timing of its inclusion and heat inactivation. Theriogenology 41:1241–1249
Farin PW, Piedrahita JA, Farin CE (2006) Errors in development of fetuses and placentas from in vitro-produced bovine embryos. Theriogenology 65:178–191
Thompson JG, Allen NW, McGowan LT, Bell AC, Lambert MG, Tervit HR (1998) Effect of delayed supplementation of fetal calf serum to culture medium on bovine embryo development in vitro and following transfer. Theriogenology 49:1239–1249
Ortega MS, Wohlgemuth S, Tribulo P, Siqueira LG, Null DJ, Cole JB, Da Silva MV, Hansen PJ (2017) A single nucleotide polymorphism in COQ9 affects mitochondrial and ovarian function and fertility in Holstein cows. Biol Reprod 96:652–663
Brevini-Gandolfi TA, Favetta LA, Mauri L, Luciano AM, Cillo F, Gandolfi F (1999) Changes in poly(A) tail length of maternal transcripts during in vitro maturation of bovine oocytes and their relation with developmental competence. Mol Reprod Dev 52:427–433
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2019 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Tríbulo, P., Rivera, R.M., Ortega Obando, M.S., Jannaman, E.A., Hansen, P.J. (2019). Production and Culture of the Bovine Embryo. In: Herrick, J. (eds) Comparative Embryo Culture. Methods in Molecular Biology, vol 2006. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9566-0_8
Download citation
DOI: https://doi.org/10.1007/978-1-4939-9566-0_8
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-4939-9565-3
Online ISBN: 978-1-4939-9566-0
eBook Packages: Springer Protocols