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Alpha-Synuclein Proximity Ligation Assay (AS-PLA) in Brain Sections to Probe for Alpha-Synuclein Oligomers

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Alpha-Synuclein

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1948))

Abstract

Alpha-synuclein oligomers are thought to be toxic mediators of Parkinson’s disease and other alpha-synucleinopathies, but their histological detection in situ in diseased brain has been a challenge in the field for some time. Here we describe a method, the alpha-synuclein proximity ligation assay (AS-PLA), to detect alpha-synuclein oligomers in paraffin-embedded brain sections. Using AS-PLA previously unobserved alpha-synuclein oligomeric pathology is revealed.

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Author information

Authors and Affiliations

  1. Montreal Neurological Institute, McGill University, Montréal, QC, Canada

    Rosalind F. Roberts

  2. Department of Physiology, Anatomy and Genetics, Oxford Parkinson’s Disease Centre, University of Oxford, Oxford, UK

    Nora Bengoa-Vergniory

  3. Division of Brain Sciences, Faculty of Medicine, Imperial College London, London, UK

    Javier Alegre-Abarrategui

Authors
  1. Rosalind F. Roberts
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  2. Nora Bengoa-Vergniory
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  3. Javier Alegre-Abarrategui
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Corresponding author

Correspondence to Javier Alegre-Abarrategui .

Editor information

Editors and Affiliations

  1. Dementia Research Institute, University College London, London, UK

    Tim Bartels

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© 2019 Springer Science+Business Media, LLC, part of Springer Nature

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Roberts, R.F., Bengoa-Vergniory, N., Alegre-Abarrategui, J. (2019). Alpha-Synuclein Proximity Ligation Assay (AS-PLA) in Brain Sections to Probe for Alpha-Synuclein Oligomers. In: Bartels, T. (eds) Alpha-Synuclein. Methods in Molecular Biology, vol 1948. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9124-2_7

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  • DOI: https://doi.org/10.1007/978-1-4939-9124-2_7

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9123-5

  • Online ISBN: 978-1-4939-9124-2

  • eBook Packages: Springer Protocols

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