Skip to main content
SpringerLink
Log in

Mouse Lung Tissue Slice Culture

  • Protocol
  • First Online:
Mouse Cell Culture

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1940))

Abstract

Precision-cut lung slices (PCLS) represent an ex vivo model widely used in visualizing interactions between lung structure and function. The major advantage of this technique is that the presence, differentiation state, and localization of the more than 40 cell types that make up the lung are in accordance with the physiological situation found in lung tissue, including the right localization and patterning of extracellular matrix elements. Here we describe the methodology involved in preparing and culturing PCLS followed by detailed practical information about their possible applications.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 189.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book
USD 249.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Similar content being viewed by others

References

  1. Konigshoff M, Uhl F, Gosens R (2011) From molecule to man: integrating molecular biology with whole organ physiology in studying respiratory disease. Pulm Pharmacol Ther 24:466–470

    Article  Google Scholar 

  2. Sanderson MJ (2011) Exploring lung physiology in health and disease with lung slices. Pulm Pharmacol Ther 24:452–465

    Article  CAS  Google Scholar 

  3. Halayko AJ, Solway J (2001) Molecular mechanisms of phenotypic plasticity in smooth muscle cells. J Appl Physiol 90:358–368

    Article  CAS  Google Scholar 

  4. Martin C, Uhlig S, Ullrich V (1996) Videomicroscopy of methacholine-induced contraction of individual airways in precision-cut lung slices. Eur Respir J 9:2479–2487

    Article  CAS  Google Scholar 

  5. Oenema TA, Maarsingh H, Smit M, Groothuis GM, Meurs H, Gosens R (2013) Bronchoconstriction induces TGF-beta release and airway remodelling in guinea pig lung slices. PLoS One 8:e65580

    Article  CAS  Google Scholar 

  6. Uhl FE, Vierkotten S, Wagner DE, Burgstaller G, Costa R et al (2015) Preclinical validation and imaging of Wnt-induced repair in human 3D lung tissue cultures. Eur Respir J 46:1150–1166

    Article  CAS  Google Scholar 

  7. Wohlsen A, Uhlig S, Martin C (2001) Immediate allergic response in small airways. Am J Respir Crit Care Med 163:1462–1469

    Article  CAS  Google Scholar 

  8. Schleputz M, Rieg AD, Seehase S, Spillner J, Perez-Bouza A et al (2012) Neurally mediated airway constriction in human and other species: a comparative study using precision-cut lung slices (PCLS). PLoS One 7:e47344

    Article  Google Scholar 

  9. Bergner A, Sanderson MJ (2002) Acetylcholine-induced calcium signaling and contraction of airway smooth muscle cells in lung slices. J Gen Physiol 119:187–198

    Article  CAS  Google Scholar 

  10. Bergner A, Sanderson MJ (2003) Airway contractility and smooth muscle Ca(2+) signaling in lung slices from different mouse strains. J Appl Physiol 95:1325–1332. discussion 1314

    Article  CAS  Google Scholar 

  11. Held HD, Martin C, Uhlig S (1999) Characterization of airway and vascular responses in murine lungs. Br J Pharmacol 126:1191–1199

    Article  CAS  Google Scholar 

  12. Khan MA, Kianpour S, Stampfli MR, Janssen LJ (2007) Kinetics of in vitro bronchoconstriction in an elastolytic mouse model of emphysema. Eur Respir J 30:691–700

    Article  CAS  Google Scholar 

  13. Van Dijk EM, Culha S, Menzen MH, Bidan CM, Gosens R (2016) Elastase-induced parenchymal disruption and airway hyper responsiveness in mouse precision cut lung slices: toward an ex vivo COPD model. Front Physiol 7:657

    PubMed  Google Scholar 

  14. Bidan CM, Veldsink AC, Meurs H, Gosens R (2015) Airway and extracellular matrix mechanics in COPD. Front Physiol 6:346

    Article  Google Scholar 

  15. Hiorns JE, Bidan CM, Jensen OE, Gosens R, Kistemaker LE et al (2016) Airway and parenchymal strains during bronchoconstriction in the precision cut lung slice. Front Physiol 7:309

    Article  Google Scholar 

  16. Kistemaker LEM, Oenema TA, Baarsma HA, Bos IST, Schmidt M et al (2017) The PDE4 inhibitor CHF-6001 and LAMAs inhibit bronchoconstriction-induced remodeling in lung slices. Am J Physiol Lung Cell Mol Physiol 313:L507–L515

    Article  Google Scholar 

  17. Alsafadi HN, Staab-Weijnitz CA, Lehmann M, Lindner M, Peschel B et al (2017) An ex vivo model to induce early fibrosis-like changes in human precision-cut lung slices. Am J Physiol Lung Cell Mol Physiol 312:L896–L902

    Article  Google Scholar 

  18. Gilpin SE, Ren X, Okamoto T, Guyette JP, Mou H et al (2014) Enhanced lung epithelial specification of human induced pluripotent stem cells on decellularized lung matrix. Ann Thorac Surg 98:1721–1729. discussion 1729

    Article  Google Scholar 

  19. Henjakovic M, Sewald K, Switalla S, Kaiser D, Muller M et al (2008) Ex vivo testing of immune responses in precision-cut lung slices. Toxicol Appl Pharmacol 231:68–76

    Article  CAS  Google Scholar 

  20. Hess A, Wang-Lauenstein L, Braun A, Kolle SN, Landsiedel R et al (2016) Prevalidation of the ex-vivo model PCLS for prediction of respiratory toxicity. Toxicol In Vitro 32:347–361

    Article  CAS  Google Scholar 

  21. De Kanter R, De Jager MH, Draaisma AL, Jurva JU, Olinga P et al (2002) Drug-metabolizing activity of human and rat liver, lung, kidney and intestine slices. Xenobiotica 32:349–362

    Article  Google Scholar 

  22. de Kanter R, Monshouwer M, Meijer DK, Groothuis GM (2002) Precision-cut organ slices as a tool to study toxicity and metabolism of xenobiotics with special reference to non-hepatic tissues. Curr Drug Metab 3:39–59

    Article  Google Scholar 

  23. Lyons-Cohen MR, Thomas SY, Cook DN, Nakano H (2017) Precision-cut mouse lung slices to visualize live pulmonary dendritic cells. J Vis Exp (122):55465

    Google Scholar 

  24. Herbert J, Thiermann H, Worek F, Wille T (2017) Precision cut lung slices as test system for candidate therapeutics in organophosphate poisoning. Toxicology 389:94–100

    Article  CAS  Google Scholar 

  25. Rosner SR, Ram-Mohan S, Paez-Cortez JR, Lavoie TL, Dowell ML et al (2014) Airway contractility in the precision-cut lung slice after cryopreservation. Am J Respir Cell Mol Biol 50:876–881

    Article  Google Scholar 

  26. Abraham T, Hogg J (2010) Extracellular matrix remodeling of lung alveolar walls in three dimensional space identified using second harmonic generation and multiphoton excitation fluorescence. J Struct Biol 171:189–196

    Article  Google Scholar 

  27. van der Strate BW, Postma DS, Brandsma CA, Melgert BN, Luinge MA et al (2006) Cigarette smoke-induced emphysema: a role for the B cell? Am J Respir Crit Care Med 173:751–758

    Article  Google Scholar 

  28. Schindelin J, Arganda-Carreras I, Frise E, Kaynig V, Longair M et al (2012) Fiji: an open-source platform for biological-image analysis. Nat Methods 9:676–682

    Article  CAS  Google Scholar 

Download references

Acknowledgment

This work is supported by a ZonMW-Vidi grant from the Netherlands Organization for Scientific Research (016.126.307), a grant for the Dutch Lung Foundation (3.2.08.014) and the China Scholarship Council (File No. 201707720065).

Author information

Authors and Affiliations

  1. Faculty of Science and Engineering, Department of Molecular Pharmacology, University of Groningen, Groningen, The Netherlands

    Xinhui Wu, Eline M. van Dijk, I. Sophie T. Bos, Loes E. M. Kistemaker & Reinoud Gosens

  2. Groningen Research Institute for Asthma and COPD, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands

    Xinhui Wu, Eline M. van Dijk, I. Sophie T. Bos, Loes E. M. Kistemaker & Reinoud Gosens

Authors
  1. Xinhui Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Eline M. van Dijk
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. I. Sophie T. Bos
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Loes E. M. Kistemaker
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Reinoud Gosens
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Reinoud Gosens .

Editor information

Editors and Affiliations

  1. Lung Health Research Centre, Department of Pharmacology and Therapeutics, University of Melbourne, Melbourne, VIC, Australia

    Ivan Bertoncello

Rights and permissions

Reprints and permissions

Copyright information

© 2019 Springer Science+Business Media, LLC, part of Springer Nature

About this protocol

Check for updates. Verify currency and authenticity via CrossMark

Cite this protocol

Wu, X., van Dijk, E.M., Bos, I.S.T., Kistemaker, L.E.M., Gosens, R. (2019). Mouse Lung Tissue Slice Culture. In: Bertoncello, I. (eds) Mouse Cell Culture. Methods in Molecular Biology, vol 1940. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9086-3_21

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-9086-3_21

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9085-6

  • Online ISBN: 978-1-4939-9086-3

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation