Abstract
Electron microscopic analysis of mineralized tissues like bone and dentin is essential for understanding of cell–cell/cell–matrix interactions, and the three-dimensional organization of these tissues. This chapter describes a few methods to process mineralized tissues obtained from different sources for ultrastructural analysis by transmission electron microscopy.
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References
Goggin PM, Zygalakis KC, Oreffo ROC, Schneider P (2016) High-resolution 3D imaging of osteocytes and computational modelling in mechanobiology: insights on bone development, ageing, health and disease. Eur Cell Mater 31:264–295
Schwarcz HP (2015) The ultrastructure of bone as revealed in electron microscopy of ion-milled sections. Semin Cell Dev Biol 46:44–50
Miltić Ž, Stolić A, Stojanović S, Najman S, Ignjatović N, Nikolić G, Trajanović M (2017) Instrumental methods and techniques for structural and physicochemical characterization of biomaterials and bone tissue: a review. Mat Sci Eng C Mater Biol Appl 79:930–949
Gruber HE (1992) Adaptations of Goldner’s Masson trichrome stain for the study of undecalcified plastic embedded bone. Biotech Histochem 67:30–34
Rungby J, Kassem M, Eriksen EF, Danscher G (1993) The von Kossa reaction for calcium deposits: silver lactate staining increases sensitivity and reduces background. Hist J 25:446–451
Reynolds ES (1963) The use of lead nitrate at high pH as an electron opaque stain in electron microscopy. J Cell Biol 17:208
Everts V, Korper W, Jansen DC, Steinfort J, Lammerse I, Heera S, Docherty AJP, Beertsen W (1999) Functional heterogeneity of osteoclasts: matrix metalloproteinases participate in osteoclastic resorption of calvarial bone but not in resorption of long bone. FASEB J 13:1219–1230
Landis WJ, Paine MC, Glimcher MJ (1978) Use of acrolein vapors for anhydrous preparation of bone tissue for electron microscopy. J Ultrastruct Res 70:171–180
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Everts, V., Niehof, A., Tigchelaar-Gutter, W., Beertsen, W. (2019). Transmission Electron Microscopy of Bone. In: Idris, A. (eds) Bone Research Protocols. Methods in Molecular Biology, vol 1914. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8997-3_32
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DOI: https://doi.org/10.1007/978-1-4939-8997-3_32
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