Abstract
The production of doubled haploid (DH) barley plants through anther culture is a very useful yet simple in vitro technique. DH plants derive from divisions of haploid microspores that have undergone a developmental switch under the appropriate conditions. The successive divisions lead to the formation of an embryo or callus rather than the formation of mature pollen grains. Plants that regenerate from these embryos are often either haploid, in which case their chromosome set can be doubled by treatment with colchicine, or spontaneous double haploids. The efficiency of DH plant production is highly variable depending on the genotype of the source material. Despite this limitation, DH plants have been widely used in breeding and research programs. Compared to conventional approaches, breeding strategies that makes use of DH plants achieve a homozygous state, allowing transgene or mutation stabilization in the genome, within a considerably shorter time, thus accelerating workflow or reducing work volume.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Guha S, Maheshwari SC (1964) In vitro production of embryos from anthers of Datura. Nature 204:497
Bourgin JP, Nitsch JP (1967) Production of haploids nicotiana from excised stamens. Annales De Physiologie Vegetale 9:377–382
Clapham D (1973) Haploid Hordeum plants from anthers in-vitro. J Plant Breed 69:142–155
Hu H (1997) In vitro induced haploids in wheat. In: Jain SM, Sopory SK, Veilleux RE (eds) In vitro haploid production in higher plants: Volume 4—Cereals. Springer Netherlands, Dordrecht, pp 73–97
Li H, Devaux P (2005) Isolated microspore culture overperforms anther culture for green plant regeneration in barley (Hordeum vulgare L.). Acta Physiol Plant 27:611–619
Lantos C, Páricsi S, Zofajova A et al (2006) Isolated microspore culture of wheat (Triticum aestivum L.) with Hungarian cultivars. Acta Biologica Szegediensis 50:31–35
Reynolds TL (1997) Pollen embryogenesis. Plant Mol Biol 33:1–10
Bonet FJ, Azbaid L, Olmedilla A (1998) Pollen embryogenesis: atavism or totipotency? Protoplasma 202:115–121
Sunderland N, Evans LJ (1980) Multicellular pollen formation in cultured barley anthers. J Exp Bot 31:501–514
Chen X-W, Cistué L, Muñoz-Amatriaín M et al (2007) Genetic markers for doubled haploid response in barley. Euphytica 158:287–294
Lazaridou T, Sistanis I, Lithourgidis A et al (2011) Response to in-vitro anther culture of F 3 families originating from high and low yielding F 2 barley (Hordeum vulgare L.) plants. Aust J Crop Sci 5:265–270
Jacquard C, Nolin F, Hécart C et al (2009) Microspore embryogenesis and programmed cell death in barley: effects of copper on albinism in recalcitrant cultivars. Plant Cell Rep 28:1329–1339
Makowska K, Oleszczuk S, Zimny A et al (2015) Androgenic capability among genotypes of winter and spring barley. Plant Breed 134:668–674
Rodríguez-Serrano M, Bárány I, Prem D et al (2012) NO, ROS, and cell death associated with caspase-like activity increase in stress-induced microspore embryogenesis of barley. J Exp Bot 63:2007–2024
Caredda S, Doncoeur C, Devaux P et al (2000) Plastid differentiation during androgenesis in albino and non-albino producing cultivars of barley (Hordeum vulgare L.). Sex Plant Reprod 13:95–104
Devaux P, Pickering R (2005) Haploids in the improvement of Poaceae. In: Don Palmer CE, Keller WA, Kasha KJ (eds) Haploids in crop improvement II. Springer Berlin Heidelberg, Berlin, Heidelberg, pp 215–242
Kahrizi D, Mahmoodi S, Khaniki GB et al (2011) Effect of genotype on androgenesis in barley (Hordeum vulgare L.). Biharean Biol 5:132–134
Shen Y, Pan G, and Lübberstedt T (2015) Haploid Strategies for Functional Validation of Plant Genes. Trends in Biotechnology. 33, 611–620
Chu CC (1978) The N6 medium and its applications to anther culture of cereal crops. In: Proceedings of symposium on plant tissue culture, Science Press, Peking, p 45–50
Zhuang J, Xu J (1983) Increasing differentiation frequencies in wheat pollen callus. In: Hu H, Vega M (eds) Cell and tissue culture techniques for cereal crop improvement. Science Press, Beijing, p 431
Dolezel J, Greilhuber J, Suda J (2007) Estimation of nuclear DNA content in plants using flow cytometry. Nat Protocol 2:2233–2244
Acknowledgments
This work was supported by the Ministry of Education, Youth and Sports, Czech Republic (Grant LO1204 from the National Program of Sustainability I.). We would like to thank the Gordon-Zeto Center for Global Education at Spelman College for supporting Tomas Vlcko and thus facilitating our collaboration. The authors are grateful to Ota Blahousek for the technical assistance.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2019 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Ohnoutkova, L., Vlcko, T., Ayalew, M. (2019). Barley Anther Culture. In: Harwood, W. (eds) Barley. Methods in Molecular Biology, vol 1900. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8944-7_4
Download citation
DOI: https://doi.org/10.1007/978-1-4939-8944-7_4
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-8942-3
Online ISBN: 978-1-4939-8944-7
eBook Packages: Springer Protocols