Abstract
Phytoplasmas are among the most recently discovered plant pathogens. They are wall-less prokaryotes restricted to phloem tissue, associated with diseases affecting several hundred plant species. The impact of phytoplasma diseases on agriculture is impressive and, at the present day, no effective curative strategy has been developed. The availability of rapid and sensitive techniques for phytoplasma detection as well as the possibility to study their relationship with the host plants is a prerequisite for the management of phytoplasma-associated diseases.
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References
Weisburg WG, Tully JG, Rose DL et al (1989) A phylogenetic analysis of the mycoplasmas: basis for their classification. J Bacteriol 171(12):6455–6467
Doi Y, Teranaka M, Yora K et al (1967) Mycoplasma- or PLT group-like microorganisms found in the phloem elements of plants infected with mulberry dwarf, potato witches’ broom, aster yellows or paulownia witches’ broom (in Japanese with English summary). Ann Phytopath Soc Japan 33:259–266
IRPCM P, Spiroplasma, WTPTG (2004) Candidatus Phytoplasma', a taxon for the wall-less, nonhelical prokaryotes that colonize plant phloem and insects. Int J Syst Evol Microbiol 54(Pt 4):1243
Bertaccini A, Duduk B (2009) Phytoplasma and phytoplasma diseases: a review of recent research. Phytopathol Mediterr 48(3):355–378
Lee IM, Gundersen-Rindal DE, Davis RE et al (2004) ‘Candidatus Phytoplasma asteris’, a novel phytoplasma taxon associated with aster yellows and related diseases. Int J Syst Evol Microbiol 54(4):1037–1048
Lee M, Martini M, Marcone C et al (2004) Classification of phytoplasma strains in the elm yellows group (16SrV) and proposal of ‘Candidatus Phytoplasma ulmi’ for the phytoplasma associated with elm yellows. Int J Syst Evol Microbiol 54(2):337–347
Marcone C, Lee IM, Davis RE et al (2000) Classification of aster yellowsgroup phytoplasmas based on combined analyses of rRNA and tuf gene sequences. Int J Syst Evol Microbiol 50(5):1703–1713
Zhao Y, Davis RE (2016) Criteria for phytoplasma 16Sr group/subgroup delineation and the need of a platform for proper registration of new groups and subgroups. Int J Syst Evol Microbiol 66(5):2121–2123
Lee IM, Davis RE, Gundersen-Rindal DE (2000) Phytoplasma: Phytopathogenic Mollicutes 1. Annu Rev Microbiol 54(1):221–255
Contaldo N, Bertaccini A, Paltrinieri S et al (2012) Axenic culture of plant pathogenic phytoplasmas. Phytopathol Mediterr 51(3):607–617
Marcone C, Neimark H, Ragozzino A et al (1999) Chromosome sizes of phytoplasmas composing major phylogenetic groups and subgroups. Phytopathology 89(9):805–810
Hogenhout SA, Loria R (2008) Virulence mechanisms of gram-positive plant pathogenic bacteria. Curr Opin Plant Biol 11(4):449–456
Weintraub PG, Beanland L (2006) Insect vectors of phytoplasmas. Annu Rev Entomol 51:91–111
Bosco D, Galetto L, Leoncini P et al (2007) Interrelationships between “Candidatus Phytoplasma asteris” and its leafhopper vectors (Homoptera: Cicadellidae). J Econ Entomol 100(5):1504–1511
Christensen NM, Axelsen KB, Nicolaisen M et al (2005) Phytoplasmas and their interactions with hosts. Trends Plant Sci 10(11):526–535
Oshima K, Ishii Y, Kakizawa S et al (2011) Dramatic transcriptional changes in an intracellular parasite enable host switching between plant and insect. PLoS One 6(8):e23242
Schaper U, Seemüller E (1984) Recolonization of the stem of apple proliferation and pear decline-diseased trees by the causal organisms in spring. Z Pflanzenkrankh Pflanzenschutz 91:608–613
Marcone C, Weintraub PG, Jones P (2009) Movement of Phytoplasmas and the development of disease in the plant. In: Genomes, plant hosts and vectors, vol 114
Pagliari L, Buoso S, Santi S et al (2017) Filamentous sieve element proteins are able to limit phloem mass flow, but not phytoplasma spread. J Exp Bot 68(13):3673–3688
Bertaccini A, Duduk B, Paltrinieri S et al (2014) Phytoplasmas and phytoplasma diseases: a severe threat to agriculture. Am J Plant Sci 5:763–1788
Valiunas V, Wang HZ, Li L et al (2015) A comparison of two cellular delivery mechanisms for small interfering RNA. Physiol Rep 3(2):e12286
Seemüller E, Garnier M, Schneider B (2002) Mycoplasmas of plants and insects. In: Molecular biology and pathogenicity of mycoplasmas. Springer, New York, pp 91–115
Osler R, Carraro L, Loi N et al (1996). Le più importanti malattie da fitoplasmi nel Friuli-Venezia Giulia: atlante. Edito da Ente regionale per la promozione e lo sviluppo dell'agricoltura del Friuli-Venezia Giulia
Musetti R, Buxa SV, De Marco F et al (2013) Phytoplasma-triggered Ca2+ influx is involved in sieve-tube blockage. MPMI 26(4):379–386
Musetti R, Favali MA (2003) Calcium localization and X-ray microanalysis in Catharanthus roseus L. infected with phytoplasmas. Micron 34:387–393
Lherminier J, Benhamou N, Larrue J et al (2003) Cytological characterization of elicitin-induced protection in tobacco plants infected by Phytophthora parasitica or phytoplasma. Phytopathology 93(10):1308–1319
Kudla J, Batistič O, Hashimoto K (2010) Calcium signals: the lead currency of plant information processing. Plant Cell 22(3):541–563
McAinsh MR, Pittman JK (2009) Shaping the calcium signature. New Phytol 181(2):275–294
van Bel AJ, Furch AC, Will T et al (2014) Spread the news: systemic dissemination and local impact of Ca2+ signals along the phloem pathway. J Exp Bot 65:1761–1787
Musetti R, Sanità di Toppi L, Martini M et al (2005) Hydrogen peroxide localization and antioxidant status in the recovery of apricot plants from European stone fruit yellows. Eur J Plant Pathol 112(1):53–61
Sánchez-Rojo S, López-Delgado HA, Mora-Herrera ME et al (2011) Salicylic acid protects potato plants-from phytoplasma-associated stress and improves tuber photosynthate assimilation. Am J Pot Res 88(2):175–183
Minato N, Himeno M, Hoshi A et al (2014) The phytoplasmal virulence factor TENGU causes plant sterility by downregulating of the jasmonic acid and auxin pathways. Sci Rep 4:1399
Punelli F, Al Hassan M, Fileccia V et al (2016) A microarray analysis highlights the role of tetrapyrrole pathways in grapevine responses to “stolbur” phytoplasma, phloem virus infections and recovered status. Physiol Mol Plant Path 93:129–137
Zimmermann MR, Schneider B, Mithöfer A et al (2015) Implications of Candidatus Phytoplasma Mali infection on phloem function of apple trees. Endocytobiosis Cell Res 26:67–75
Ji X, Gai Y, Zheng C et al (2009) Comparative proteomic analysis provides new insights into mulberry dwarf responses in mulberry (Morus alba L.). Proteomics 9(23):5328–5339
Hren M, Nikolić P, Rotter A et al (2009) 'Bois noir'phytoplasma induces significant reprogramming of the leaf transcriptome in the field grown grapevine. BMC Genomics 10(1):1
Taheri F, Nematzadeh G, Zamharir MG et al (2011) Proteomic analysis of the Mexican lime tree response to “Candidatus Phytoplasma aurantifolia” infection. Mol BioSystems 7(11):3028–3035
Bertamini M, Grando MS, Muthuchelian K et al (2002a) Effect of phytoplasmal infection on photosystem II efficiency and thylakoid membrane protein changes in field grown apple (Malus pumila) leaves. Physiol Mol Plant Path 61(6):349–356
Bertamini M, Nedunchezhian N, Tomasi F et al (2002b) Phytoplasma [Stolbur-subgroup bois noir-BN] infection inhibits photosynthetic pigments, ribulose-1, 5-bisphosphate carboxylase and photosynthetic activities in field grown grapevine (Vitis vinifera L. cv. Chardonnay) leaves. Physiol Mol Plant Path 61(6):357–366
Junqueira A, Bedendo I, Pascholati S (2004) Biochemical changes in corn plants infected by the maize bushy stunt phytoplasma. Physiol Mol Plant Path 65(4):181–185
Lepka P, Stitt M, Moll E et al (1999) Effect of phytoplasmal infection on concentration and translocation of carbohydrates and amino acids in periwinkle and tobacco. Physiol Mol Plant Path 55(1):59–68
Zafari S, Niknam V, Musetti R et al (2012) Effect of phytoplasma infection on metabolite content and antioxidant enzyme activity in lime (Citrus aurantifolia). Acta Physiol Plant 34(2):561–568
Maust BE, Espadas F, Talavera C et al (2003) Changes in carbohydrate metabolism in coconut palms infected with the lethal yellowing phytoplasma. Phytopathology 93(8):976–981
Pagliari L, Martini M, Loschi A et al (2016) Looking inside phytoplasma-infected sieve elements: a combined microscopy approach using Arabidopsis thaliana as a model plant. Micron 89:87–97
Bertamini M, Grando MS, Nedunchezhian N (2003) Effects of phytoplasma infection on pigments, chlorophyll-protein complex and photosynthetic activities in field grown apple leaves. Biol Plant 47(2):237–242
Favali MA, Sanità di Toppi L, Vestena C et al (2001) Phytoplasmas associated with tomato stolbur disease. Acta Hortic 551:93–99
Margaria P, Palmano S (2011) Response of the Vitis vinifera L. cv. ‘Nebbiolo’ proteome to Flavescence dorée phytoplasma infection. Proteomics 11(2):212–224
Santi S, Grisan S, Pierasco A et al (2013) Laser microdissection of grapevine leaf phloem infected by stolbur reveals site-specific gene responses associated to sucrose transport and metabolism. Plant Cell Environ 36(2):343–355
Zhong BX, Shen YW (2004) Accumulation of pathogenesis-related Type-5 like proteins in Phytoplasma infected garland chrysanthemum Chrysanthemum coronarium. Acta Biochim Biophys Sin 36(11):773–779
van Loon LC, van Strien EA (1999) The families of pathogenesis-related proteins, their activities, and comparative analysis of PR-1 type proteins. Physiol Mol Plant Path 55(2):85–97
Boonrod K, Munteanu B, Jarausch B et al (2012) An immunodominant membrane protein (imp) of 'Candidatus Phytoplasma mali' binds to plant actin. Mol Plant-Microbe Interact 25(7):889–895
Galetto L, Bosco D, Balestrini R et al (2011) The major antigenic membrane protein of “Candidatus Phytoplasma asteris” selectively interacts with ATP synthase and actin of leafhopper vectors. PLoS One 6(7):e22571
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Pagliari, L., Musetti, R. (2019). Phytoplasmas: An Introduction. In: Musetti, R., Pagliari, L. (eds) Phytoplasmas. Methods in Molecular Biology, vol 1875. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8837-2_1
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DOI: https://doi.org/10.1007/978-1-4939-8837-2_1
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