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A Method for Isolation of Stromal Vascular Fraction Cells in a Clinically Relevant Time Frame

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Adipose-Derived Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1773))

Abstract

There is increasing interest in the clinical applications of adipose-derived stem cells (ASCs) and the stromal vascular fraction (SVF) based on promising preclinical data. As adipose-derived therapeutics begin to translate into the clinical setting, it is important to maintain patient safety as well as uniformity in technique. Here, we describe a method for isolation of stromal vascular fraction cells in a clinically relevant time frame. Analytical laboratory techniques are mentioned, but respective protocols are not provided here.

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References

  1. Ude CC, Sulaiman SB, Min-Hwei N et al (2014) Cartilage regeneration by chondrogenic induced adult stem cells in osteoarthritic sheep model. PLoS One 9(6):e98770

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Planat-Bernard V, Silvestre JS, Cousin B et al (2004) Plasticity of human adipose lineage cells towards endothelial cells: physiological and therapeutic perspectives. Circulation 109:656–663

    Article  Google Scholar 

  3. Naderi N, Wilde C, Haque T et al (2014) Adipogenic differentiation of adipose-derived stem cells in a 3-dimensional spheroid culture (microtissue): implications for the reconstructive surgeon. J Plast Reconstr Aesthet Surg 67(12):1726–1734

    Article  PubMed  Google Scholar 

  4. Zuk PA, Zhu M, Mizuno H et al (2001) Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng 7:211–229

    Article  CAS  PubMed  Google Scholar 

  5. Yoshimura K, Sato K, Aoi N et al (2008) Cell-assisted lipotransfer for cosmetic breast augmentation: supportive use of adipose-derived stem/stromal cells. Aesthet Plast Surg 32(1):48–55

    Article  Google Scholar 

  6. Rehmam J, Traktuev D, Li J et al (2004) Secretion of angiogenic and antiapoptotic factors by human adipose stromal cells. Circulation 109:1292–1298

    Article  Google Scholar 

  7. Kapur SK, Katz AJ (2013) Review of the adipose derived stem cell secretome. Biochimie 95:2222–2228

    Article  CAS  PubMed  Google Scholar 

  8. Matsumoto D, Sato K, Gonda K et al (2006) Cell-assisted lipotansfer: supportive use of human adipose-derived stem cells for soft tissue augmentation with lipoinjection. Tissue Eng 12(12):3375–3383

    Article  CAS  PubMed  Google Scholar 

  9. Lee HC, An SG, Lee HW et al (2012) Safety and effect of adipose tissue-derived stem cell implantation in patients with critical limb ischemia. Circ J 76:1750–1760

    Article  CAS  PubMed  Google Scholar 

  10. Salibian AA, Widgerow AD, Abrouk M et al (2013) Stem cells in plastic surgery: a review of current clinical and translational applications. Arc Plast Surg 40:666–675

    Article  Google Scholar 

  11. Michalek J, Moster R, Lukac L et al (2015) Autologous adipose tissue-derived stromal vascular fraction cells application in patients with osteoarthritis. Cell Transplant. https://doi.org/10.3727/096368915X686760

  12. Gautam M, Fujita D, Kimura K et al (2015) Transplantation of adipose tissue-derived stem cells improves cardiac contractile function and electrical stability in a rat myocardial infarction model. J Mol Cell Cardiol 81:139–149

    Article  CAS  PubMed  Google Scholar 

  13. Shah FS, Wu X, Dietrich M et al (2013) A non-enzymatic method for isolating human adipose-derived stromal stem cells. Cytotherapy 15:979–985

    Article  CAS  PubMed  Google Scholar 

  14. Markarian FM, Frey GZ, Silveira MD et al (2014) Isolation of adipose-derived stem cells: a comparison among different methods. Biotechnol Lett 36:693–702

    Article  CAS  PubMed  Google Scholar 

  15. Baptista LS, do Amaral RJ, Carias RB et al (2009) An alternative method for the isolation of mesenchymal stromal cells derived from lipoaspirate samples. Cytotherapy 11(6):706–715

    Article  CAS  PubMed  Google Scholar 

  16. Yoshimura K, Shiguera T, Matsumoto D et al (2006) Characterization of freshly isolated and cultured cells derived from the fatty and fluid portions of liposuction aspirates. J Cell Physiol 208:64–76

    Article  CAS  PubMed  Google Scholar 

  17. Wünsch E, Heidrich HG (1963) On the qualitative determination of collagenase. Z Physiol Chem 333:149

    Article  Google Scholar 

  18. McCarthy RC, Spurlin B, Wright MJ et al (2008) Development and characterization of a collagen degradation assay to assess purified collagenase used in islet isolation. Transplant Proc 40:339

    Article  CAS  PubMed  Google Scholar 

  19. Aronowitz JA, Ellenhorn JD (2013) Adipose stromal vascular fraction isolation: a head-to-head comparison of four commercial cell separation systems. Plast Reconstr Surg 132(6):932e–939e

    Article  CAS  PubMed  Google Scholar 

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None of the authors have a financial interest in any of the products, devices, or drugs mentioned in this chapter.

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Correspondence to Joel A. Aronowitz .

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Aronowitz, J.A., Lockhart, R.A., Hakakian, C.S. (2018). A Method for Isolation of Stromal Vascular Fraction Cells in a Clinically Relevant Time Frame. In: Bunnell, B.A., Gimble, J.M. (eds) Adipose-Derived Stem Cells. Methods in Molecular Biology, vol 1773. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7799-4_2

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  • DOI: https://doi.org/10.1007/978-1-4939-7799-4_2

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7797-0

  • Online ISBN: 978-1-4939-7799-4

  • eBook Packages: Springer Protocols

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