Abstract
Murine models of obesity or reduced adiposity are a valuable resource for understanding the role of adipocyte dysfunction in metabolic disorders. Adipose tissue stromal vascular cells or primary adipocytes derived from murine adipose tissue and grown in culture are essential tools for studying the mechanisms underlying adipocyte development and function. Herein, we describe methods for the isolation, expansion, and long-term storage of murine adipose-derived stromal/stem cells along with protocols for inducing adipogenesis in this cell population or isolating the adipose stromal vascular fraction cells for flow cytometric analysis.
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Acknowledgment
This work is supported by the National Institutes of Health (NIDDK, 1R01DK099625, Z.E.F.). This work used the Cell Biology and Bioimaging Core facility at Pennington Biomedical Research Center that is supported in part by COBRE (NIH 8P20-GM103528) and NORC (NIH 2P30-DK072476) center grants from the National Institutes of Health.
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Kilroy, G., Dietrich, M., Wu, X., Gimble, J.M., Floyd, Z.E. (2018). Isolation of Murine Adipose-Derived Stromal/Stem Cells for Adipogenic Differentiation or Flow Cytometry-Based Analysis. In: Bunnell, B.A., Gimble, J.M. (eds) Adipose-Derived Stem Cells. Methods in Molecular Biology, vol 1773. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7799-4_11
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DOI: https://doi.org/10.1007/978-1-4939-7799-4_11
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