Abstract
Encapsulation methodologies allow the protection of bacteriophages for overcoming critical environmental conditions. Moreover, they improve the stability and the controlled delivery of bacteriophages which is of great innovative value in bacteriophage therapy. Here, two different encapsulation methodologies of bacteriophages are described using two biocompatible materials: a lipid cationic mixture and a combination of alginate with the antacid CaCO3. To perform bacteriophage encapsulation, a purified lysate highly concentrated (around 1010–1011 pfu/mL) is necessary, and to dispose of a specific equipment. Both methodologies have been successfully applied for encapsulating Salmonella bacteriophages with different morphologies. Also, the material employed does not modify the antibacterial action of bacteriophages. Moreover, both technologies can also be adapted to any bacteriophage and possibly to any delivery route for bacteriophage therapy.
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Acknowledgement
This work was supported by grants from La Caixa and the Associació Catalana d’Universitats Públiques (2010ACUP00300), AGAUR-ACCIÓ-Generalitat de Catalunya (2010VALOR00114), the Ministerio de Ciencia e Innovación de España (BFU2011-23478), and AGAUR-Generalitat de Catalunya (2014SGR572 and 2014SGR80). We are grateful to the Servei de Microscòpia of the Universitat Autònoma de Barcelona for its support. ICN2 acknowledges the support of the Spanish MINECO through the Severo Ochoa Centres of Excellence Programme, under Grant SEV-2013-0295.
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Cortés, P., Cano-Sarabia, M., Colom, J., Otero, J., Maspoch, D., Llagostera, M. (2018). Nano/Micro Formulations for Bacteriophage Delivery. In: Azeredo, J., Sillankorva, S. (eds) Bacteriophage Therapy. Methods in Molecular Biology, vol 1693. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7395-8_20
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DOI: https://doi.org/10.1007/978-1-4939-7395-8_20
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