Abstract
Natural bacteriophages (present in the microbiome) and those applied as therapeutic agents may interact with mammalian cells and tissues. Adhesion interactions may define bacteriophage pharmacokinetics and resulting efficiency of bacteriophage agents in therapeutic applications by shaping bacteriophage homing to tissues and organs. Here we propose protocols for testing direct adhesion of bacteriophages or bacteriophage proteins to mammalian cells (in vitro). We further propose an animal model for investigation of accumulation/homing of bacteriophages in tissues (in vivo).
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References
Scarpellini E, Ianiro G, Attili F, Bassanelli C, De Santis A, Gasbarrini A (2015) The human gut microbiota and virome: potential therapeutic implications. Dig Liver Dis 47:1007–1012
Górski A, Dąbrowska K, Hodyra-Stefaniak K, Borysowski J, Międzybrodzki R, Weber-Dąbrowska B (2015) Phages targeting infected tissues: novel approach to phage therapy. Future Microbiol 10(2):199–204
Hodyra-Stefaniak K, Miernikiewicz P, Drapała J, Drab M, Jończyk-Matysiak E, Lecion D, Kaźmierczak Z, Beta W, Majewska J, Harhala M, Bubak B, Kłopot A, Górski A, Dąbrowska K (2015) Mammalian host-versus-phage immune response determines phage fate in vivo. Sci Rep 5:14802
Payne RJ, Phil D, Jansen VA (2000) Phage therapy: the peculiar kinetics of self-replicating pharmaceuticals. Clin Pharmacol Ther 68(3):225–230
Barr JJ, Auro R, Furlan M, Whiteson KL, Erb ML, Pogliano J, Stotland A, Wolkowicz R, Cutting AS, Doran KS, Salamon P, Youle M, Rohwer F (2013) Bacteriophage adhering to mucus provide a non-host-derived immunity. Proc Natl Acad Sci U S A 110:10771–10776
Edwards RA, McNair K, Faust K, Raes J, Dutilh BE (2016) Computational approaches to predict bacteriophage-host relationships. FEMS Microbiol Rev 40:258–272
Ceglarek I, Piotrowicz A, Lecion D, Miernikiewicz P, Owczarek B, Hodyra K, Harhala M, Górski A, Dąbrowska K (2013) A novel approach for separating bacteriophages from other bacteriophages using affinity chromatography and phage display. Sci Rep 3:3220
Kaźmierczak Z, Piotrowicz A, Owczarek B, Hodyra K, Miernikiewicz P, Lecion D, Harhala M, Górski A, Dąbrowska K (2014) Molecular imaging of T4 phage in mammalian tissues and cells. Bacteriophage 4:e28364
Sambrook J, Russell DW (eds) (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory, New York, NY
Adams M (1957) Bacteriophages. Interscience Publishers INC., New York, NY
Acknowledgment
This work was supported by the National Science Centre in Poland, grant UMO-2012/05/E/NZ6/03314 and grant UMO-2015/19/N/NZ4/03609.
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Kaźmierczak, Z., Dąbrowska, K. (2018). Interaction of Bacteriophages with Mammalian Cells. In: Azeredo, J., Sillankorva, S. (eds) Bacteriophage Therapy. Methods in Molecular Biology, vol 1693. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7395-8_10
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DOI: https://doi.org/10.1007/978-1-4939-7395-8_10
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