Abstract
OligoSTORM and OligoDNA-PAINT meld the Oligopaint technology for fluorescent in situ hybridization (FISH) with, respectively, Stochastic Optical Reconstruction Microscopy (STORM) and DNA-based Point Accumulation for Imaging in Nanoscale Topography (DNA-PAINT) to enable in situ single-molecule super-resolution imaging of nucleic acids. Both strategies enable ≤20 nm resolution and are appropriate for imaging nanoscale features of the genomes of a wide range of species, including human, mouse, and fruit fly (Drosophila).
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Acknowledgments
The authors would like to thank members of the Wu, Zhuang, and Yin laboratories for extensive conversations and experience. This work was supported by grants from the NIH to the laboratories of C.-t W. (GM085169, DP1GM106412, RM1HG008525), X.Z. (R01GM105637), and P.Y. (1R01EB018659, 1-U01-MH106011), X.Z. is a Howard Hughes Medical Institute investigator. In addition, B.J.B. and A.N.B. were supported by Damon Runyon Postdoctoral Fellowships.
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Beliveau, B.J. et al. (2017). In Situ Super-Resolution Imaging of Genomic DNA with OligoSTORM and OligoDNA-PAINT. In: Erfle, H. (eds) Super-Resolution Microscopy. Methods in Molecular Biology, vol 1663. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7265-4_19
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DOI: https://doi.org/10.1007/978-1-4939-7265-4_19
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