Abstract
Genome editing is driving a revolution in the biomedical sciences that carries the promise for future treatments of genetic diseases. The CRISPR/Cas9 system of RNA-guided genome editing has been successfully applied to modify the genome of a wide spectrum of organisms. We recently showed that this technique can be combined with in vivo electroporation to inhibit the function of genes of interest in somatic cells of the developing chicken embryo. We present here a simplified version of the previously described technique that leads to effective gene loss-of-function.
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Acknowledgments
We thank Dr. Daniel Sieiro for critical reading of the manuscript. This work was supported by grants from the National Health and Medical Research Council (NHMRC, Australia) to C.M. and N.V. and by the Programme Avenir Lyon Saint-Etienne (PALSE) from the University of Lyon to C.M and V.M. The Australian Regenerative Medicine Institute is supported by grants from the State Government of Victoria and the Australian Government. The NeuroMyoGene Institute is supported by grants from the Association Française contre les Myopathies (AFM).
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Morin, V., Véron, N., Marcelle, C. (2017). CRISPR/Cas9 in the Chicken Embryo. In: Sheng, G. (eds) Avian and Reptilian Developmental Biology. Methods in Molecular Biology, vol 1650. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7216-6_7
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DOI: https://doi.org/10.1007/978-1-4939-7216-6_7
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