Abstract
The C19 steroid 1,4-androstadiene-3,17-dione (androstadienedione, ADD) is an added value product used as a synthon in the pharmaceutical industry for the commercial production of corticosteroids, mineralocorticoids, oral contraceptives, and other pharmaceutical steroids. Phytosterol biotransformation catalyzed by microbial whole cells is actually a very well-established research area in white biotechnology. The protocol below provides detailed information on ADD production by the mutant CECT 8331 of Mycobacterium smegmatis mc2155 using phytosterols as raw material in a lab scale. This protocol describes the bioconversion of phytosterols into ADD in a single fermentation step.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Znidarssic-Plazl P, Plazl I (2010) Development of a continuous steroid biotransformation process and product extraction within microchannel system. Catal Today 157:315–320
García JL, Uhía I, Galán B (2012) Catabolism and biotechnological applications of cholesterol degrading bacteria. Microb Biotechnol 5:679–699
Malaviya A, Gomes J (2008) Androstenedione production by biotransformation of phytosterols. Bioresour Technol 99:6725–6737
Donova MV, Egorova OV (2012) Microbial steroid transformations: current state and prospects. Appl Microbiol Biotechnol 94:1423–1447
Donova MV, Gulevskaya SA, Dovbnya DV, Puntus IF (2005) Mycobacterium sp. mutant strain producing 9α-hydroxyandrostenedione from sitosterol. Appl Microbiol Biotechnol 67:671–678
Andor A, Jekkel A, Hopwood DA, Jeanplong F, Ilkoy E, Kónya A, Kurucz I, Ambrus G (2006) Generation of useful insertionally blocked sterol degradation pathway mutants of fast-growing mycobacteria and cloning, characterization, and expression of the terminal oxygenase of the 3-ketosteroid 9α-hydroxylase in Mycobacterium smegmatis mc2155. Appl Environ Microbiol 72:6554–6559
García JL, Uhía I, Galán B (2014) Mutantes recombinantes selectivos de Mycobacterium smegmatis mc2155 y su uso para la producción de 1,4-androsten-3,17-diona o 4-androsten-3,17-diona a partir de esteroles naturales. Spanish Patent N° P201430272.
Kutney JP (2003) Process for fermentation of phytosterols to androstadienedione. WO 2003/064674
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2017 Springer Science+Business Media LLC
About this protocol
Cite this protocol
García-Fernández, J., Martínez, I., Fernández-Cabezón, L., Felpeto-Santero, C., García, JL., Galán, B. (2017). Bioconversion of Phytosterols into Androstadienedione by Mycobacterium smegmatis CECT 8331. In: Barredo, JL., Herráiz, I. (eds) Microbial Steroids. Methods in Molecular Biology, vol 1645. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7183-1_15
Download citation
DOI: https://doi.org/10.1007/978-1-4939-7183-1_15
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7182-4
Online ISBN: 978-1-4939-7183-1
eBook Packages: Springer Protocols