Abstract
A lateral flow biosensor (LFB) is introduced for the detection of single nucleotide polymorphisms (SNPs). The assay is composed of two steps: circular strand displacement reaction and lateral flow biosensor detection. In step 1, the nucleotide at SNP site is recognized by T4 DNA ligase and the signal is amplified by strand displacement DNA polymerase, which can be accomplished at a constant temperature. In step 2, the reaction product of step 1 is detected by a lateral flow biosensor, which is a rapid and cost effective tool for nuclei acid detection. Comparing with conventional methods, it requires no complicated machines. It is suitable for the use of point of care diagnostics. Therefore, this simple, cost effective, robust, and promising LFB detection method of SNP has great potential for the detection of genetic diseases, personalized medicine, cancer related mutations, and drug-resistant mutations of infectious agents.
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References
Watanabe H, Sawabu N, Songur Y et al (1996) Detection of K-ras point mutations at codon 12 in pure pancreatic juice for the diagnosis of pancreatic cancer by PCR-RFLP analysis. Pancreas 12:18–24
Gunderson KL, Steemers FJ, Lee G et al (2005) A genome-wide scalable SNP genotyping assay using microarray technology. Nat Genet 37:549–554
Lindroos K, Sigurdsson S, Johansson K et al (2002) Multiplex SNP genotyping in pooled DNA samples by a four-colour microarray system. Nucleic Acids Res 30:e70
Bernard PS, Lay MJ, Wittwer CT (1998) Integrated amplification and detection of the C677T point mutation in the methylenetetrahydrofolate reductase gene by fluorescence resonance energy transfer and probe melting curves. Anal Biochem 255:101–107
Wabuyele MB, Farquar H, Stryjewski W et al (2003) Approaching real-time molecular diagnostics: single-pair fluorescence resonance energy transfer (spFRET) detection for the analysis of low abundant point mutations in K-ras oncogenes. J Am Chem Soc 125:6937–6945
Huang Y, Zhang Y-L, Xu X et al (2009) Highly Specific and Sensitive Electrochemical Genotyping via Gap Ligation Reaction and Surface Hybridization Detection. J Am Chem Soc 131:2478–2480
Guo Q, Yang X, Wang K et al (2009) Sensitive fluorescence detection of nucleic acids based on isothermal circular strand-displacement polymerization reaction. Nucleic Acids Res 37:e20
Li JH, Chu X, Liu YL et al (2005) A colorimetric method for point mutation detection using high-fidelity DNA ligase. Nucleic Acids Res 33:E168
Hashimoto M, Barany F, Soper SA (2006) Polymerase chain reaction/ligase detection reaction/hybridization assays using flow-through microfluidic devices for the detection of low-abundant DNA point mutations. Biosens Bioelectron 21:1915–1923
Lie P, Liu J, Fang Z et al (2012) A lateral flow biosensor for detection of nucleic acids with high sensitivity and selectivity. Chem Commun 48:236–238
Fang Z, Ge C, Zhang W et al (2011) A lateral flow biosensor for rapid detection of DNA-binding protein c-jun. Biosens Bioelectron 27:192–196
Liu G, Mao X, Phillips JA et al (2009) Aptamer-nanoparticle strip biosensor for sensitive detection of cancer cells. Anal Chem 81:10013–10018
Fang ZY, Huang J, Lie PC et al (2010) Lateral flow nucleic acid biosensor for Cu2+ detection in aqueous solution with high sensitivity and selectivity. Chem Commun 46:9043–9045
Breivik J, Meling GI, Spurkland A et al (1994) K-ras mutation in colorectal cancer: relations to patient age, sex and tumour location. Br J Cancer 69:367–371
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Zeng, L., Xiao, Z. (2017). A Lateral Flow Biosensor for the Detection of Single Nucleotide Polymorphisms. In: Prickril, B., Rasooly, A. (eds) Biosensors and Biodetection. Methods in Molecular Biology, vol 1572. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6911-1_27
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DOI: https://doi.org/10.1007/978-1-4939-6911-1_27
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