Abstract
Length is a functional parameter of telomeres, the nucleoprotein structures that protect chromosome ends. The availability of highly specific, high affinity probes for telomeric repeat sequences allowed the development of quantitative approaches aimed at measuring telomere length directly on chromosomes or in interphase nuclei. Here, we describe a general method for telomere quantitative FISH on metaphase chromosomes and discuss its most common applications in research.
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Acknowledgments
Work in the Londoño laboratory is supported by “La Ligue contre le Cancer”, the “Fondation pour la Recherche Medicale, FRM”, the “Association pour la Recherche sur le Cancer, ARC”. I. O-G. was the recipient of a PhD fellowship from Agence Nationale de la Recherche (ANR) and ARC.
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Ourliac-Garnier, I., Londoño-Vallejo, A. (2017). Telomere Length Analysis by Quantitative Fluorescent in Situ Hybridization (Q-FISH). In: Songyang, Z. (eds) Telomeres and Telomerase. Methods in Molecular Biology, vol 1587. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6892-3_3
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DOI: https://doi.org/10.1007/978-1-4939-6892-3_3
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