Abstract
Dendritic cells (DCs) are central to initiate antigen-specific immunity and tolerance. The in vivo development and distribution of DCs are now better understood even in nonlymphoid tissues [1]. Atherosclerosis is a chronic inflammatory disease of blood vessels and DCs are highly enriched in the intimal area of the aorta, which is predisposed to develop atherosclerosis. Previously, we were the first to show antigen presenting DCs and their subsets in the aorta [2, 3]. Here, we discuss several useful methods to characterize not only DCs but also other immune cells in steady state and atherosclerotic aorta. These comprise multiparameter flow cytometry strategies including intracellular staining and cell sorting, en face immunohistochemistry of DCs and regulatory T cells (Tregs), and Oil Red O staining of atherosclerotic lesions in the aorta.
The original version of this chapter was revised. The erratum to this chapter is available at: DOI 10.1007/978-1-4939-6786-5_31
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Acknowledgment
This work was supported by grants from National Research Foundation of Korea (GRN-2013S1A2A2035348) (JHC and CC) and Canadian Institutes of Health Research (CIHR, MOP-125933 and HIG-133050) (CC). CC holds a Chercheur-Boursier Junior of Fonds de recherche du Québec-Santé (FRQS)—Société québécoise d’hypertension artérielle (SQHA) and CIHR New Investigator Salary Award.
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Yun, T.J., Lee, J.S., Shim, D., Choi, JH., Cheong, C. (2017). Isolation and Characterization of Aortic Dendritic Cells and Lymphocytes in Atherosclerosis. In: Clausen, B., Laman, J. (eds) Inflammation. Methods in Molecular Biology, vol 1559. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6786-5_29
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DOI: https://doi.org/10.1007/978-1-4939-6786-5_29
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