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Introduction: History of SH2 Domains and Their Applications

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SH2 Domains

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1555))

Abstract

The Src Homology 2 (SH2) domain is the prototypical protein interaction module that lies at the heart of phosphotyrosine signaling. Since its serendipitous discovery, there has been a tremendous advancement in technologies and an array of techniques available for studying SH2 domains and phosphotyrosine signaling. In this chapter, we provide a glimpse of the history of SH2 domains and describe many of the tools and techniques that have been developed along the way and discuss future directions for SH2 domain studies. We highlight the gist of each chapter in this volume in the context of: the structural biology and phosphotyrosine binding; characterizing SH2 specificity and generating prediction models; systems biology and proteomics; SH2 domains in signal transduction; and SH2 domains in disease, diagnostics, and therapeutics. Many of the individual chapters provide an in-depth approach that will allow scientists to interrogate the function and role of SH2 domains.

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Acknowledgments

We are incredibly grateful to all the authors who contributed to this volume. We thank the series editor, John Walker, for his continuous encouragement and providing constructive comments. We also thank Joshua Jadwin, Piers Nash, and Bruce Mayer for their support. Finally, we would like to extend our thanks to David Casey and Anna Rakovsky of Springer USA, and L. Sujitha of Spi Global for their help in the compilation of this book. This study was partly supported by grant CA1154966 from the National Institutes of Health and Quest for CURES (QFC) grant from the Leukemia and Lymphoma Society (to K.M.).

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Authors and Affiliations

  1. Broad Institute of Harvard and MIT, 415 Main St., 5175 JJ, Cambridge, MA, 02142, USA

    Bernard A. Liu

  2. Raymond and Beverly Sackler Laboratory of Genetics and Molecular Medicine, Department of Genetics and Genome Sciences, University of Connecticut School of Medicine, 400 Farmington Ave., Farmington, CT, 06030, USA

    Kazuya Machida

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  1. Bernard A. Liu
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  2. Kazuya Machida
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Correspondence to Bernard A. Liu or Kazuya Machida .

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Editors and Affiliations

  1. Department of Genetics and Genome Sciences, University of Connecticut School of Medicine, Farmington, Connecticut, USA

    Kazuya Machida

  2. Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA

    Bernard A. Liu

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Liu, B.A., Machida, K. (2017). Introduction: History of SH2 Domains and Their Applications. In: Machida, K., Liu, B. (eds) SH2 Domains. Methods in Molecular Biology, vol 1555. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6762-9_1

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  • DOI: https://doi.org/10.1007/978-1-4939-6762-9_1

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