Abstract
Cellular senescence is a unique process of normal physiology, from embryonic development to aging, also known for its association with a broad range of pathological conditions. Therefore a reliable model of cellular senescence remains an indispensable tool for the investigation of senescence-associated changes and human disease. Here we describe a model of HT1080 fibrosarcoma cells with an inducible senescence phenotype. These cells are equipped with the lac repressor and exogenous p21 under the control of a lac repressor regulated promoter. The senescent phenotype is induced in these cells by isopropyl-β-d-thiogalactopyranoside (IPTG)-inducible expression of senescence-associated cell cycle inhibitor p21Waf1/Cip1/Sdi1.
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Acknowledgments
This work was supported by NIH grant P20GM109091 (M.S., G.S., E.V.B.) and ACS grant IRG-13-043-01 (E.V.B.).
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Shtutman, M., Chang, BD., Schools, G.P., Broude, E.V. (2017). Cellular Model of p21-Induced Senescence. In: Nikiforov, M. (eds) Oncogene-Induced Senescence. Methods in Molecular Biology, vol 1534. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6670-7_3
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DOI: https://doi.org/10.1007/978-1-4939-6670-7_3
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