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Methods for Initial Characterization of Campylobacter jejuni Bacteriophages

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Campylobacter jejuni

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1512))

Abstract

Here we describe an initial characterization of Campylobacter jejuni bacteriophages by host range analysis, genome size determination by pulsed-field gel electrophoresis, and receptor-type identification by screening mutants for phage sensitivity.

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References

  1. Kropinski AM, Mazzocco A, Waddell TE, et al. (2009) Enumeration of bacteriophages by double agar overlay plaque assay. In: Clokie MRJ, Kropinski AM (eds) Bacteriophages: methods and protocols, Volume 1: Isolation, characterization, and interactions. p 69

    Google Scholar 

  2. Janež N, Kokošin A, Zaletel E et al (2014) Identification and characterization of new Campylobacter group III phages of animal origin. FEMS Microbiol Lett 359:64–71

    Article  PubMed  Google Scholar 

  3. Hansen VM, Rosenquist H, Baggesen DL et al (2007) Characterization of Campylobacter phages including analysis of host range by selected Campylobacter Penner serotypes. BMC Microbiol 18(7):90

    Article  Google Scholar 

  4. El-Shibiny A, Connerton PL, Connerton IF (2005) Enumeration and diversity of campylobacters and bacteriophages isolated during the rearing cycles of free-range and organic chickens. Appl Environ Microbiol 71:1259–1266

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Sørensen MCH, Gencay YE, Birk T et al (2015) Primary isolation strain determines both phage type and receptors recognised by Campylobacter jejuni bacteriophages. PLoS One 10:e0116287

    Article  PubMed  PubMed Central  Google Scholar 

  6. Baldvinsson SB, Sørensen MCH, Vegge CS et al (2014) Campylobacter jejuni motility is required for infection of the flagellotropic bacteriophage F341. Appl Environ Microbiol 80:7096–7106

    Article  PubMed  PubMed Central  Google Scholar 

  7. Holst Sørensen MC, van Alphen LB, Fodor C et al (2012) Phase variable expression of capsular polysaccharide modifications allows Campylobacter jejuni to avoid bacteriophage infection in chickens. Front Cell Infect Microbiol 2:11

    PubMed  Google Scholar 

  8. Sørensen MCH, van Alphen LB, Harboe A, Li J et al (2011) Bacteriophage F336 recognizes the capsular phosphoramidate modification of Campylobacter jejuni NCTC11168. J Bacteriol 193:6742–6749

    Article  PubMed  PubMed Central  Google Scholar 

  9. Coward C, Grant AJ, Swift C et al (2006) Phase-variable surface structures are required for infection of Campylobacter jejuni by bacteriophages. Appl Environ Microbiol 72:4638–4647

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Brathwaite KJ, Siringan P, Moreton J et al (2013) Complete genome sequence of universal bacteriophage host strain Campylobacter jejuni subsp. jejuni PT14. Genome Announc 1:e00969–13

    Article  PubMed  PubMed Central  Google Scholar 

  11. Lingohr E, Frost S, Johnson RP. (2009) Determination of bacteriophage genome size by pulsed-field gel electrophoresis. In: Clokie MRJ, Kropinski AM (eds) Bacteriophages: methods and protocols, Volume 2: Molecular and applied aspects. p 19

    Google Scholar 

  12. Javed MA, Ackermann HW, Azeredo J et al (2014) A suggested classification for two groups of Campylobacter myoviruses. Arch Virol 159:181–190

    Article  CAS  PubMed  Google Scholar 

  13. Ackermann HW (2009) Basic phage electron microscopy. In Clokie MRJ, Kropinski AM (eds) Bacteriophages: methods and protocols, Volume 1: Isolation, characterization, and interactions. p 113

    Google Scholar 

  14. Green MR, Sambrook J (2012) Molecular cloning: a laboratory manual, 4th edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY

    Google Scholar 

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Correspondence to Martine Camilla Holst Sørensen .

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Sørensen, M.C.H., Gencay, Y.E., Brøndsted, L. (2017). Methods for Initial Characterization of Campylobacter jejuni Bacteriophages. In: Butcher, J., Stintzi, A. (eds) Campylobacter jejuni. Methods in Molecular Biology, vol 1512. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6536-6_9

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  • DOI: https://doi.org/10.1007/978-1-4939-6536-6_9

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6534-2

  • Online ISBN: 978-1-4939-6536-6

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