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A Modified Protocol for the Isolation of Primary Human Hepatocytes with Improved Viability and Function from Normal and Diseased Human Liver

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Hepatocyte Transplantation

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1506))

Abstract

Successful hepatocyte isolation is critical for continued development of cellular transplantation. However, most tissue available for research is from diseased liver and the results of hepatocyte isolation from such tissue are inferior compared to normal tissue. Here we describe a modified method, combining the use of Liberase and N-acetylcysteine (NAC), for the isolation of primary human hepatocytes with high viability from normal and diseased liver.

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Acknowledgment

This work was supported by the Medical Research Council.

Author information

Authors and Affiliations

  1. National Institute for Health Research (NIHR) Biomedical Research Unit and Centre for Liver Research, University of Birmingham, Birmingham, UK

    David C. Bartlett & Philip N. Newsome

  2. The Liver Unit, Queen Elizabeth Hospital Birmingham, Birmingham, UK

    David C. Bartlett & Philip N. Newsome

Authors
  1. David C. Bartlett
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  2. Philip N. Newsome
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Corresponding authors

Correspondence to David C. Bartlett or Philip N. Newsome .

Editor information

Editors and Affiliations

  1. Department of Surgery, University of Leipzig, Leipzig, Germany

    Peggy Stock

  2. Department of Surgery, University of Leipzig, Leipzig, Germany

    Bruno Christ

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Bartlett, D.C., Newsome, P.N. (2017). A Modified Protocol for the Isolation of Primary Human Hepatocytes with Improved Viability and Function from Normal and Diseased Human Liver. In: Stock, P., Christ, B. (eds) Hepatocyte Transplantation. Methods in Molecular Biology, vol 1506. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6506-9_4

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  • DOI: https://doi.org/10.1007/978-1-4939-6506-9_4

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6504-5

  • Online ISBN: 978-1-4939-6506-9

  • eBook Packages: Springer Protocols

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