Abstract
Mechanical forces, including hydrodynamic shear, hydrostatic pressure, compression, tension, and friction, can have stimulatory effects on cartilage synthesis in tissue engineering systems. Bioreactors capable of exerting forces on cells and tissue constructs within a controlled culture environment are needed to provide appropriate mechanical stimuli. In this chapter, we describe the construction, assembly, and operation of a mechanobioreactor providing simultaneous dynamic shear and compressive loading on developing cartilage tissues to mimic the rolling and squeezing action of articular joints. The device is suitable for studying the effects of mechanical treatment on stem cells and chondrocytes seeded into three-dimensional scaffolds.
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References
Martel-Pelletier J, Boileau C, Pelletier J-P et al (2008) Cartilage in normal and osteoarthritis conditions. Best Pract Res Clin Rheumatol 22:351–384
Kiani C, Chen L, Wu YJ et al (2002) Structure and function of aggrecan. Cell Res 12:19–32
Archer CW, Dowthwaite GP, Francis-West P (2003) Development of synovial joints. Birth Defects Res C Embryo Today 69:144–155
Schulz RM, Bader A (2007) Cartilage tissue engineering and bioreactor systems for the cultivation and stimulation of chondrocytes. Eur Biophys J 36:539–568
Kisiday JD, Jin M, DiMicco MA et al (2004) Effects of dynamic compressive loading on chondrocyte biosynthesis in self-assembling peptide scaffolds. J Biomech 37:595–604
Waldman SD, Spiteri CG, Grynpas MD et al (2004) Long-term intermittent compressive stimulation improves the composition and mechanical properties of tissue-engineered cartilage. Tissue Eng 10:1323–1331
Mouw JK, Connelly JT, Wilson CG et al (2007) Dynamic compression regulates the expression and synthesis of chondrocyte-specific matrix molecules in bone marrow stromal cells. Stem Cells 25:655–663
Terraciano V, Hwang N, Moroni L et al (2007) Differential response of adult and embryonic mesenchymal progenitor cells to mechanical compression in hydrogels. Stem Cells 25:2730–2738
Pelaez D, Huang C-YC, Cheung HS (2009) Cyclic compression maintains viability and induces chondrogenesis of human mesenchymal stem cells in fibrin gel scaffolds. Stem Cells Dev 18:93–102
Haugh MG, Meyer EG, Thorpe SD et al (2011) Temporal and spatial changes in cartilage-matrix-specific gene expression in mesenchymal stem cells in response to dynamic compression. Tissue Eng A 17:3085–3093
Li Z, Yao S-J, Alini M et al (2010) Chondrogenesis of human bone marrow mesenchymal stem cells in fibrin–polyurethane composites is modulated by frequency and amplitude of dynamic compression and shear stress. Tissue Eng A 16:575–584
Grad S, Loparic M, Peter R et al (2012) Sliding motion modulates stiffness and friction coefficient at the surface of tissue engineered cartilage. Osteoarthritis Cartilage 20:288–295
Huang AH, Baker BM, Ateshian GA et al (2012) Sliding contact loading enhances the tensile properties of mesenchymal stem cell-seeded hydrogels. Eur Cell Mater 24:29–45
Shahin K, Doran PM (2012) Tissue engineering of cartilage using a mechanobioreactor exerting simultaneous mechanical shear and compression to simulate the rolling action of articular joints. Biotechnol Bioeng 109:1060–1073
Pourmohammadali H, Chandrashekar N, Medley JB (2013) Hydromechanical stimulator for chondrocyte-seeded constructs in articular cartilage tissue engineering applications. Proc Inst Mech Eng H 227:310–316
Bonassar LJ, Grodzinsky AJ, Frank EH et al (2001) The effect of dynamic compression on the response of articular cartilage to insulin-like growth factor-I. J Orthop Res 19:11–17
Mauck RL, Hung CT, Ateshian GA (2003) Modeling of neutral solute transport in a dynamically loaded porous permeable gel: implications for articular cartilage biosynthesis and tissue engineering. J Biomech Eng 125:602–614
Kelly T-AN, Ng KW, Wang CC-B et al (2006) Spatial and temporal development of chondrocyte-seeded agarose constructs in free-swelling and dynamically loaded cultures. J Biomech 39:1489–1497
Abdel-Sayed P, Darwiche SE, Kettenberger U et al (2014) The role of energy dissipation of polymeric scaffolds in the mechanobiological modulation of chondrogenic expression. Biomaterials 35:1890–1897
Acknowledgements
This work was funded by the Australian Research Council (ARC). We are grateful to Russell Cail for assistance with design of the mechanobioreactor and John Matiossa for device fabrication and workshop services.
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Shahin, K., Doran, P.M. (2015). Shear and Compression Bioreactor for Cartilage Synthesis. In: Doran, P. (eds) Cartilage Tissue Engineering. Methods in Molecular Biology, vol 1340. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2938-2_16
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DOI: https://doi.org/10.1007/978-1-4939-2938-2_16
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2937-5
Online ISBN: 978-1-4939-2938-2
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