Abstract
High-throughput sequencing of short cDNA tags, or RNA-Seq, has become a staple of genome-wide gene expression studies in plants. RNA-Seq libraries necessarily contain tags that correspond to the mRNA-poly(A) junction, or polyadenylation site, and thus may be mined for data that can help study alternative polyadenylation. This report presents a simple, rapid, and inexpensive method for preparing strand-specific RNA-Seq libraries from varying quantities of total RNA.
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Acknowledgements
This work was supported by the National Science Foundation (awards IOS-0817818 and MCB-1243849). The author thanks the staff of AGTC for many helpful discussions and for much patience, and Carol Von Lanken for excellent technical and administrative assistance.
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Hunt, A.G. (2015). A Rapid, Simple, and Inexpensive Method for the Preparation of Strand-Specific RNA-Seq Libraries. In: Hunt, A., Li, Q. (eds) Polyadenylation in Plants. Methods in Molecular Biology, vol 1255. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2175-1_17
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DOI: https://doi.org/10.1007/978-1-4939-2175-1_17
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