Abstract
Maintenance of mitochondrial membrane integrity is essential for mitochondrial function and neuronal viability . Apoptotic stimulus or calcium overload leads to mitochondrial permeability transition pore (mPTP ) opening and induces mitochondrial swelling , a common feature of mitochondrial membrane permeabilization. The first phenomenon can be evaluated in cells loaded with the dye calcein -AM quenched by cobalt , and mitochondrial swelling can be detected by electron microscopy through the analysis of mitochondrial membrane integrity. Here, we describe a live cell imaging assay to detect mitochondrial permeability transition and the development of a detailed analysis of morphological and ultrastructural changes that mitochondria undergo during this process.
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Acknowledgments
This work was supported by grants from the Basal Center of Excellence in Aging and Regeneration (CONICYT—PFB 12/2007) and FONDECYT N° 1120156 to NCI, and a predoctoral fellowship from CONICYT to MSA.
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Arrázola, M.S., Inestrosa, N.C. (2015). Monitoring Mitochondrial Membranes Permeability in Live Neurons and Mitochondrial Swelling Through Electron Microscopy Analysis. In: Lossi, L., Merighi, A. (eds) Neuronal Cell Death. Methods in Molecular Biology, vol 1254. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2152-2_7
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DOI: https://doi.org/10.1007/978-1-4939-2152-2_7
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