Abstract
The use of flow cytometry in vertebrate nervous tissues is hampered by the morphological complexity and high level of interconnectivity intrinsic to their cellular constituents. Here we describe a simplified procedure for the identification and quantitative analysis of neural cells by flow cytometry based on the isolation and immunolabeling of fresh cell nuclei . We have applied this procedure for the quantitative analysis of apoptosis and DNA synthesis in the embryonic brain .
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References
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Acknowledgements
This study was supported by grants from the “Ministerio de Ciencia e Innovación” (SAF2012-38316) and “Fundación Areces” (CIVP16A1815).
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López-Sánchez, N., Frade, J.M. (2015). Flow Cytometric Analysis of DNA Synthesis and Apoptosis in Central Nervous System Using Fresh Cell Nuclei . In: Lossi, L., Merighi, A. (eds) Neuronal Cell Death. Methods in Molecular Biology, vol 1254. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2152-2_3
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DOI: https://doi.org/10.1007/978-1-4939-2152-2_3
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2151-5
Online ISBN: 978-1-4939-2152-2
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