Abstract
Single-molecule localization-based super-resolution microscopy can be performed with regular, bright, and photostable organic fluorophores. We review a concept termed direct stochastic optical reconstruction microscopy (dSTORM), which operates conventional fluorophores as photoswitches and provides an optical resolution of ~20 nm. We introduce the principle of dSTORM, illustrate experimental schemes, and discuss approaches for data analysis.
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Acknowledgments
The authors thank Marina Dietz, Franziska Fricke, and Bianca Nouvertné for providing super-resolution images. This work was supported by the Bundesministerium für Bildung und Forschung (FORSYS program, grant number 0315262).
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Endesfelder, U., Heilemann, M. (2015). Direct Stochastic Optical Reconstruction Microscopy (dSTORM). In: Verveer, P. (eds) Advanced Fluorescence Microscopy. Methods in Molecular Biology, vol 1251. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2080-8_14
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DOI: https://doi.org/10.1007/978-1-4939-2080-8_14
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