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Mast Cell and Basophil Cell Lines: A Compendium

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Basophils and Mast Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1192))

Abstract

Mast cells and basophils play a crucial role during type I hypersensitivity reactions. However, despite efforts to elucidate their role in the pathogenesis of allergy and inflammation, our understanding of mast cell and basophil biology is still relatively scarce. The practical difficulty in obtaining a sufficient number of purified primary cells from biological samples has slowed down the process of reaching a full understanding of the physiological role of these functionally similar cell types. The establishment of several immortalized cell lines has been a useful tool to establish and perform sophisticated laboratory protocols that are impractical using primary cells. Continuous cell lines have been extensively used to investigate the allergen/IgE-mediated cell activation, to elucidate the degranulation dynamics, to investigate structural and functional properties of the high-affinity receptor (FcεRI), and to test cell-stabilizing compounds. In this chapter we review the most widely used and better characterized mast cell and basophil cell lines, highlighting their advantages and drawbacks. It must be pointed out, however, that while cell lines represent a useful in vitro tool due to their easy manipulability and reduced culture costs, they often show aberrant characteristics which are not fully representative of primary cell physiology; results obtained with such cells therefore must be interpreted with due care.

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Author information

Authors and Affiliations

  1. School of Pharmacy and Biomedical Sciences, University of Central Lancashire, Maudland Building 240, Preston, PR1 2HE, UK

    Egle Passante

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  1. Egle Passante
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Correspondence to Egle Passante .

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Editors and Affiliations

  1. Medway School of Pharmacy, The University of Kent, Chatham Maritime, Kent, United Kingdom

    Bernhard F. Gibbs

  2. Division of Molecular and Cellular Science, The School of Pharmacy, The University of Nottingham, Nottingham, United Kingdom

    Franco H. Falcone

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Passante, E. (2014). Mast Cell and Basophil Cell Lines: A Compendium. In: Gibbs, B., Falcone, F. (eds) Basophils and Mast Cells. Methods in Molecular Biology, vol 1192. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1173-8_8

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  • DOI: https://doi.org/10.1007/978-1-4939-1173-8_8

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1172-1

  • Online ISBN: 978-1-4939-1173-8

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