Abstract
The analysis of human B cell populations of the blood relies on the expression of surface markers, mainly CD19, CD24, CD38, and CD27. According to these surface markers, three main B cell subsets can be identified in the blood: immature transitional B cells (CD19+CD24highCD38high), naïve B cells (CD19+CD24intCD38int) that have not encountered an antigen, and memory B cells (CD19+CD27+). To date, human B cells with regulatory functions have been essentially described within the CD24highCD38high transitional B cell subset. CD24highCD38high transitional B cells are able to produce interleukin 10 (IL-10) and to regulate in vitro Th1 and Th17 CD4+ T cell activation. Here, we provide the methods to analyze and purify the CD24highCD38high transitional B cell subset for further in vitro experiments. We also provide a reliable method to detect B cell IL-10 production using intracellular cytokine staining.
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de Masson, A., Le Buanec, H., Bouaziz, JD. (2014). Purification and Immunophenotypic Characterization of Human B Cells with Regulatory Functions. In: Vitale, G., Mion, F. (eds) Regulatory B Cells. Methods in Molecular Biology, vol 1190. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1161-5_4
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DOI: https://doi.org/10.1007/978-1-4939-1161-5_4
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-1161-5
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