Abstract
Doubled haploid (DH) techniques using immature microspores or embryos rescued in vitro following interspecific hybridization are important tools for breeding wheat and barley and for generating populations for gene mapping purposes. Here, we describe methods being used for the successful production of spring wheat and barley DHs in Australia.
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Abbreviations
- BA:
-
6-Benzyladenine
- IAA:
-
Indole-3-acetic acid
- 2,4-D:
-
2,4-Dichlorophenoxyacetic acid
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Broughton, S., Sidhu, P.K., Davies, P.A. (2014). In vitro Culture for Doubled Haploids: Tools for Molecular Breeding. In: Fleury, D., Whitford, R. (eds) Crop Breeding. Methods in Molecular Biology, vol 1145. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0446-4_14
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DOI: https://doi.org/10.1007/978-1-4939-0446-4_14
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