Abstract
With the advent of recombinant DNA technology, our knowledge of the transcription and replication processes of positive-strand RNA viruses has increased profoundly. A major breakthrough in this field has been the development of full-length cDNA clones from which infectious RNA transcripts can be made. Such clones have now been constructed for members of the Picorna-(Racaniello and Baltimore, 1981), Biomo- (Ahlquist et al., 1984), Alpha- (Rice et al., 1987) and Flaviviridae (Lai et al., 1991; Rice et al., 1989). Unfortunately, the enormous length of the coronavirus genome has hampered the construction of a full-length cDNA clone so far.
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Keywords
- Infectious Bronchitis Virus
- Mouse Hepatitis Virus
- Negative Strand
- Brome Mosaic Virus
- Feline Infectious Peritonitis Virus
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van der Most, R.G., Spaan, W.J.M. (1995). Coronavirus Replication, Transcription, and RNA Recombination. In: Siddell, S.G. (eds) The Coronaviridae. The Viruses. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1531-3_2
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