Abstract
Saponins are common plant secondary metabolites (glycosylated triterpenoid or steroid molecules) which are found in a wide range of dicotyledonous plant species, and also in some monocots1–5. Many saponins have been demonstrated to have potent antifungal activities and often occur in healthy plants at levels which are anticipated to be toxic to saponin-sensitive fungi6. This has led to speculation that saponins may act as pre-formed determinants of resistance to fungal attack. Van Etten et al.7 have proposed the term “phytoanticipin” to distinguish pre-formed antimicrobial substances from those induced de novo in response to pathogen attack (phytoalexins). By this definition saponins fall into the category of phytoanticipins. However, in some instances the concentrations of saponins have been reported to increase in response to microbial attack8,9.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
References
S.B. Mahato, S. Sarkar, and G. Poddar, Triterpenoid saponins, Phytochemistry 27: 3037 (1988).
S.B. Mahato and A.K. Nandy, Triterpenoid saponins discovered between 1987 and 1989, Phytochemistry 30: 1357 (1991).
K. Hostettmann, M. Hostettmann, and A. Marston, Saponins, Methods in Plant Biochemistry 7: 435 (1991).
K.R. Price, I.T. Johnson, and G.R. Fenwick, The chemistry and biological significance of saponins in food and feedingstuffs, CRC Crit. Rev. Food Sci. Nutr. 26: 27 (1987).
G.R. Fenwick, K.R. Price, C. Tsukamota, and K. Okubo, Saponins, in: Toxic Substances in Crop Plants, J.P. D’Mello, C.M. Duffus, and J.H. Duffus, eds., The Royal Society of Cambridge, Cambridge, pp. 285–327, (1992).
F. Schönbeck and E. Schlösser, Preformed substances as plant protectants, in: Physiological Plant Pathology, R. Heitefuss and P.H. Williams, eds., Springer-Verlag, Berlin, pp. 653–678 (1976).
H.D. VanEtten, J.W. Mansfield, J.A. Bailey, and E.E. Farmer, Two classes of plant antibiotics: phytoalexins versus “phytoanticipins”, The Plant Cell 9: 1191 (1994).
D.J. McCance and R.B. Drysdale, Production of tomatine and rishitin in tomato plants inoculated with Fusarium oxysporum f.sp. lycopersici, Physiol. Mol. Plant Pathol. 7: 221 (1975).
G.F. Pegg, G.F. and S. Woodward, Synthesis and metabolism of a-tomatine in tomato isolines in relation to resistance to Verticillium albo-atrum, Physiol. Mol. Plant. Pathol. 28: 187 (1986).
A.D. Bangham, and R.W. Home, Action of saponin on biological membranes, Nature, 196: 952 (1962).
R.R. Dourmaskin, R.M. Dougherty, and R.J.C. Harris, Electron microscopic observations on Rous sarcoma virus and cell membranes, Nature 194: 1116 (1962).
J.G. Roddick, and R.B. Drysdale, Destablization of liposome membranes by the steroidal glycoalkaloid a-tomatine, Phytochemistry 23: 543 (1984).
C.C. Steel and R.B. Drysdale, Electrolyte leakage from plant and fungal tissues and disruption of liposome membranes by a-tomatine, Phytochemistry 27: 1025 (1988).
H.U. Lüning and E. Schlösser, Role of saponins in antifungal resistance V. Enzymatic activation of avenacosides, Z. Pflanzenkrankh. Pflanzenschutz 82: 699 (1975).
R. Tschesche and W. Wiemann, Desgluco-avenacosid-A und -B, biologisch aktive Nuatigeninglycoside, Chem. Ber. 110: 2416 (1977).
A. Nisius, The stromacentre in Avena plastids and aggregation of β-glucosidase responsible for the activation of oat-leaf saponins, Planta 173: 474 (1988).
S. Gus-Mayer, H. Brunner, H.A.W. Schneider-Poetsch, and W. Rüdiger, Avenacosidase from oat: purification, sequence analysis and biochemical characterisation of a new member of the BGA family of β-glucosidases, Plant Mol. Biol. 26: 909 (1994).
S. Gus-Mayer, H. Brunner, H.A.W. Schneider-Poetsch, F. Lottspeich, C. Eckerskorn, R. Grimm, and W. Rüdiger, The amino acid sequence previously attributed to a protein kinase or a TCP1-related molecular chaperone and co-purified with phytochrome is a β-glucosidase, FEBS Letts. 347: 51 (1994).
E. Schlösser, Role of saponins in antifungal resistance. III. Tomatin dependant development of fruit rot organisms on tomato fruits, Z. Pflanzenkrankh. Pflanzenschutz 82: 476 (1975).
P.A. Arneson and R.D. Durbin, The sensitivity of fungi to α-tomatine, Phytopathology 58: 536 (1968).
A.E. Osbourn, P. Bowyer, G. Bryan, P. Lunness, B.R. Clarke, and M.J. Daniels, Detoxification of plant saponins by fungi, in: Advances in Molecular Genetics of Plant-Microbe Interactions, M.J. Daniels, J.A. Downie, and A.E. Osbourn, eds., Kluwer, Dordrecht, Vol.3, pp. 215–221 (1994).
E.M. Turner, An enzymic basis for pathogen specificity in Ophiobolus graminis, J. Exp. Bot. 12: 169 (1961).
W.M.L. Crombie, L. Crombie, J.B. Green, and J.A. Lucas, Pathogenicity of take-all fungus to oats: its relationship to the concentration and detoxification of the four avenacins, Phytochemistry 25: 2075 (1986).
A.E. Osbourn, B.R. Clarke, J.M. Dow, J.M., and M.J. Daniels, Partial characterization of avenacinase from Gaeumannomyces graminis var. avenae, Physiol. Mol. Plant Pathol. 38: 301 (1991).
P. Bowyer, B.R. Clarke, P. Lunness, M.J. Daniels, and A.E. Osbourn, Host range of a plant pathogenic fungus determined by a saponin detoxifying enzyme, Science 267: 371 (1995).
R.D. Durbin and J.F. Uchytil, Purification and properties of a fungal β-glucosidase acting on a-tomatine, Biochim. Biophys. Acta 191: 176 (1969).
J.E. Ford, DJ. McCance, and R.B. Drysdale, The detoxification of a-tomatine by Fusarium oxysporum f.sp. lycopersici, Phytochemistry 16: 545 (1977).
K. Verhoeff and J.I. Liem, Toxicity of tomatine to Botrytis cinerea, in relation to latency, Phytopath. Z. 82: 333 (1975).
L. Crombie, W.M.L. Crombie, and D.A. Whiting, Isolation of avenacins A-1, A-2, B-1 and B-2 from oat roots: structures of their aglycones, the avenestergenins, J. Chem. Soc., Chem. Commun. 244: 246 (1984).
L. Crombie, W.M.L. Crombie, and D.A. Whiting, Structures of the oat root resistance factors to take-all disease, avenacins A-1, A-2, B-1 and B-2 and their companion substances, J. Chem. Soc. Perkin Trans. I: 1917 (1986).
W.M.L. Crombie and L. Crombie, Distribution of the avenacins A-1, A-2, B-1 and B-2 in oat roots: their fungicidal activity towards take-all fungus, Phytochemistry 25: 2069 (1986).
E.M. Turner, The nature of the resistance of oats to the take-all fungus. III. Distribution of the inhibitor in oat seeedlings, J. Exp. Bot. 11: 403 (1960).
R.H. Goodwin and B.M. Pollock, Studies on mots. I. Properties and distribution of fluorescent constituents in Avena roots, Am. J. Bot. 4: 516 (1954).
A.E. Osbourn, B.R. Clarke, P. Lunness, P.R. Scott and M.J. Daniels, An oat species lacking avenacin is susceptible to infection by Gaeumannomyces graminis var. tritici, Physiol. Mol. Plant Pathol. 45: 457 (1994).
J.V. Maizel, H.J. Burkhardt, and H.K. Mitchell, Avenacin, an antimicrobial substance isolated from Avena sativa, Biochemistry 3: 424 (1964).
H.U. Lüning and E. Schlösser, Saponine in Avena saliva, Angewandte Botanik 50: 49 (1976).
T.D. Fontaine, J.S. Ard, and R.M. Ma, Tomatidine, a steroid secondary amine, J. Amer. Chem. Soc. 73: 878 (1951).
J.M. Henson, N.K. Blake, and A.L. Pilgeram, Transformation of Gaeumannomyces graminis to benomyl resistance, Curr. Genet. 14: 113 (1988).
B. Henrissat, A classification of glycosyl hydrolases based on amino acid sequence similarities, Biochem. J. 280: 309 (1991).
C.C. Barnett, R.M. Berka, and T. Fowler, Cloning and amplification of the gene encoding an extracellular β-glucosidase from Trichoderma reesei: evidence for improved rates of saccharification of cellulosic substrates, Bio/Technology 9: 562 (1991).
M. Machida, I. Ohtsuki, S. Fukui, and I. Yamashita, Nucleotide sequence of Saccharomycopsis fibuligera genes for extracellular β-glucosidases as expressed in Saccharomyces cerevisiae, Appl. Env. Microbiol. 54: 3147 (1988).
C. Kohchi and A. Tohe, Nucleotide sequence of Candida pelliculosa β-glucosidase gene, Nucleic Acids Res. 13: 6273 (1985).
E. Bause and G. Legler, Isolation and structure of a tryptic glycopeptide from the active site of βglucosidase A3 from Aspergillus wentii, Biochim. Biophys. Acta 626: 459 (1980).
P.A. Arneson and R.D. Durbin, Studies on the mode of action of tomatine as a fungitoxic agent, Plant Physiol. 1968, 43, 683–686.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1996 Springer Science+Business Media New York
About this chapter
Cite this chapter
Osbourn, A.E., Bowyer, P., Daniels, M.J. (1996). Saponin Detoxification by Plant Pathogenic Fungi. In: Waller, G.R., Yamasaki, K. (eds) Saponins Used in Traditional and Modern Medicine. Advances in Experimental Medicine and Biology, vol 404. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1367-8_45
Download citation
DOI: https://doi.org/10.1007/978-1-4899-1367-8_45
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4899-1369-2
Online ISBN: 978-1-4899-1367-8
eBook Packages: Springer Book Archive