Abstract
α-Tocopherol, the biologically most active form of Vitamin E, decreases in a concentration dependent way, proliferation of smooth muscle cells. At the same concentrations (10 – 50 μM) it induces inhibition of protein kinase C activity. Proliferation and protein kinase C inhibition by α-tocopherol, the lack of inhibition by β-tocopherol and the prevention by ß-tocopherol indicate that the mechanism involved is not related to the radical-scavenging properties of these two molecules, which are essentially equal. All-rac-α-tocopherol is less potent than RRR-α-tocopherol on smooth muscle cell proliferation. probucol (10 – 50 μM), a potent lipophilic antioxidant, does not inhibit smooth muscle cell proliferation and protein kinase C activity. In rabbit studies, atherosclerosis was induced by a 2% cholesterol-containing, vitamin E poor-diet. Six different groups of rabbits each were received vitamin E, probucol; probucol plus vitamin E. After 4 weeks, aortas were analysed for protein kinase C activity. Their media smooth muscle cells exhibited an increase in protein kinase C activity. Vitamin E fully prevented cholesterol induced atherosclerotic lesions and the induction of protein kinase C activity. Probucol was not effective in preventing both cholesterol induced atherosclerotic lesions and the induction of protein kinase C activity. These results show that the protective effect of vitamin E against hypercholesterolemic atherosclerosis is not produced by an other antioxidant such as probucol and therefore may not be linked to the antioxidant properties of this vitamin. The effects observed at the level of smooth muscle cells ex-vivo and cell culture suggest an involvement of signal transduction events on the onset of atherosclerosis. It is at this level that the protective effect of vitamin E against atherosclerosis is exerted.
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Özer, N.K. et al. (1998). Prevention of Atherosclerosis by α-Tocopherol in Smooth Muscle Cells by a Mechanism Involving Signal Transduction Modulation. In: Özben, T. (eds) Free Radicals, Oxidative Stress, and Antioxidants. NATO ASI Series, vol 296. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-2907-8_29
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DOI: https://doi.org/10.1007/978-1-4757-2907-8_29
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