Abstract
In recent years attempts have been made to equate in vivo senescence with the finite lifespan demonstrated by human diploid cells. in vitro (1). While preliminary evidence indicates that it may be possible to use cultured cells as an in vitro model system for cellular aging, several critical questions need to be resolved before any true parallel can be drawn between the in vitro phenomenon and the in vivo process.
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References
Cristofalo, V. J. 1972. Animal cell cultures as a model system for the study of aging. Adv. Gerontol. 4: 45.
Hayflick, L. 1965. The limited in vitro lifetime of human diploid cell strains. Exp. Cell Res. 37: 614.
Hay, R.J. 1970, Cell strain senescence in vitro: -`cell culture anomaly or an expression of a fundamental inability of normal cells to survive and proliferate. In “Aging in Cell and Tissue Culture”, ( E. Holeckova and V.J. Cristofalo, eds.), Plenum Press, New York.
Dell’Orco, R.T., Mertens, J.G. and Kruse, P.F. Jr. 1973. Doubling potential, calendar time, and senescence of human diploid cells in culture. Exp. Cell Res. 77: 356.
Dell’Orco, R.T., Mertens, J.G. and Kruse, P.F. Jr. 1974. Doubling potential, calendar time, and donor age of human diploid cells in culture. Exp. Cell Res. 84: 363
Kruse, P.F. Jr., Whittle, W.L. and Miedema, E. 1969. Mitotic and nonmitotic multiple-layered perfusion cultures. J. Cell Biol. 42: 113.
Tobey, R.A., Crissman,’H.A. and Kraemer, P.M. 1972, A method for comparing effects of different synchronizing protocols on mammalian cell cycle traverse. J. Cell Biol. 54: 638.
Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall, R.J 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem, 193: 265.
Dell’Orco, R.T., Crissman, H.A., Steinkamp, J.A. and Kraemer, P.M. Submitted for publication, 1974. Population analysis of arrested human diploid fibroblasts by flow microfluorometry.
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Dell’Orco, R.T. (1975). The Use of Arrested Populations of Human Diploid Fibroblasts for the Study of Senescence in Vitro . In: Cristofalo, V.J., Holečková, E. (eds) Cell Impairment in Aging and Development. Advances in Experimental Medicine and Biology, vol 53. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-0731-1_4
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DOI: https://doi.org/10.1007/978-1-4757-0731-1_4
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