Abstract
The opportunity to accurately localize radioactive substances in tissues at the cellular level became available with the introduction of relatively low energy beta-emitters, combined with the techniques of autoradiography. The major advantage offered by autoradiography is the ability to infer the location of individual molecules on the basis of the proximity of a reduced silver grain to this molecule. Therefore, attempts to increase the resolution of the method have utilized approaches that bring the two sites closer together. This paper will review some of the factors that relate to the theory and application of autoradiography when used with both light and electron microscopy.
Recipient of a Research Career Development Award (DE-K3-9053) from the United States Public Health Service.
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References
Berg, W.F., and Webb, J.H., in Mees, C.E.K. (editor), The Theory of the Photographic Process, Macmillan, New York, 1962, p. 147.
Liquier-Milward, J., Nature 177: 619 (1956).
O’Brien, R.T., and George, L.A., Nature 183: 1461 (1959).
Caro, L.G., J. Cell Biol. 15: 189 (1962).
Bachmann, L., and Salpeter, M. M., in Bahr, G. F., and Zeitler, E.H. (editors), Quantitative Electron Microscopy, Williams and Wilkins, Baltimore, 1965, p. 303.
Pelc, S. R., J. Royal Microscop. Soc. 81: 131 (1963).
Salpeter, M.M., and Bachmann, L., J. Cell Biol. 22: 469 (1964).
Young, B. A., and Kopriwa, B. M., J. Histochem. Cytochem. 12: 438 (1964).
Caro, L.G., and Van Tubergen, R.P., J. Cell Biol. 15: 173 (1962).
Caro, L.G., in Prescott, D.M. (editor), Methods in Cell Physiology, Vol. 1, Academic Press, New York, 1964, p. 327.
Revel, J. P., and Hay, E. D., Z. Zellforsch. Mikroskop. Anat. 61: 110 (1963).
Caro, L.G., and Palade, G.E., J. Cell Biol. 20: 473 (1964).
Van Heyningen, H. E., Anat. Rec. 148: 485 (1964).
Ross, R., in Montagna, W., and Billingham, R.E. (editors), Advances in Biology of Skin: Wound Healing, Pergamon Press, London, 1964, p. 144.
Pelc, S.R., Coombes, J.D., and Budd, G.C., Exp. Cell Res. 24: 192 (1961).
Hay, E.D., and Revel, J.P., Develop. Biol. 7: 152 (1963).
Godman, G.C., and Lane, N., J. Cell Biol. 21: 353 (1964).
Ross, R., and Benditt, E.P., J. Cell Biol. 27: 83 (1965).
Stein, O., and Gross, J., Exp. Cell Res. 31: 208 (1964).
Revel, J.P., and Hay, E.B., Exp. Cell Res. 25: 474 (1962).
Van Tubergen, R.P., J. Biophys. Biochem. Cytol. 9: 219 (1961).
Granboulan, P., J. Royal Microscop. Soc. 81: 165 (1963).
Ross, R., and Benditt, E.P., J. Cell Biol. 15: 99 (1962).
Ross, R., in Leblond, C. P., and Warren, K.B. (editors), The Use of Radio-autography in Investigating Protein Synthesis, Symposia of the International Society for Cell Biology, Vol. 4, Academic Press, New York, 1965, p. 273.
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© 1966 New England Nuclear Corporation
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Ross, R. (1966). Electron Microscope Autoradiography. In: Rothchild, S. (eds) Advances in Tracer Methodology. Springer, New York, NY. https://doi.org/10.1007/978-1-4684-8625-4_14
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DOI: https://doi.org/10.1007/978-1-4684-8625-4_14
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