Summary
The oxygen consumption rate \(\left( \dot{Q}{{O}_{2}} \right)\) of EMT6/Ro-cells cultured as monolayers was investigated as a function of their proliferative status. The transition of these cells from the exponential to the plateau growth phase was associated with a continuous decrease in \(\dot{Q}{{O}_{2}}\) per single cell. This decrease can partially be attributed to a corresponding decline in cellular volume. In addition, the \(\dot{Q}{{O}_{2}}\) per cell volume was also reduced during the passage of the cells through the growth phase described. The results lead to the conclusion that a reduction in cellular volume and factors which are still unknown may contribute to the metabolic changes observed.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Freyer, J.P. and Sutherland, R.M. (1985). A reduction in the in situ rates of oxygen and glucose comsumption of cells in EMT6/Ro spheroids during growth. J. Cell. Physiol. 124, 516–524.
Freyer, J.P., Tustanoff, E., Franko, A.J. and Sutherland, R.M. (1984). In situ oxygen consumption rates of cells in V-79 multicellular spheroids during growth. J. Cell. Physiol. 118, 53–61.
Kallinowski, F., Schaefer, C. and Vaupel, P. (1986). Oxygen consumption rate of ascites tumor cells during growth. 34th Annual Meeting Radiation Research Society, Las Vegas, NV.
Mueller-Klieser, W., Zander, R. and Vaupel, P. (1986). A new photometric method for oxygen consumption measurements in cell suspensions. J. Appl. Physiol. 61, 449–455.
Rockwell, S.C., Kallman, R.F. and Fajardo, L.F. (1972). Characteristics of a serially transplanted mouse mammary tumor and its tissue-culture-adapted derivative. J. Natl. Cancer Inst. 49, 735–749.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1987 Plenum Press, New York
About this chapter
Cite this chapter
Walenta, S., Mueller-Klieser, W. (1987). Oxygen Consumption Rate of Tumour Cells as a Function of their Proliferative Status. In: Silver, I.A., Silver, A. (eds) Oxygen Transport to Tissue IX. Advances in Experimental Medicine and Biology, vol 215. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-7433-6_47
Download citation
DOI: https://doi.org/10.1007/978-1-4684-7433-6_47
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-7435-0
Online ISBN: 978-1-4684-7433-6
eBook Packages: Springer Book Archive