Abstract
Much of the attention and interest in modern molecular biology is focussed on the regulation of gene expression. Factors influencing or mediating such regulation are often best studied using gene fusions. Gene fusions can be defined as DNA constructions (performed in vitro or in vivo) that result in the coding sequences from one gene (reporter) being transcribed and/or translated under the direction of the controlling sequences of another gene (controller). Most frequently, the reporter encodes an enzyme. Use of reporter enzymes can greatly enhance the sensitivity of gene fusion measurements, and can also facilitate many types of analysis.
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Jefferson, R.A. (1988). Plant Reporter Genes: The Gus Gene Fusion System. In: Setlow, J.K. (eds) Genetic Engineering. Genetic Engineering, vol 10. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-7081-3_13
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DOI: https://doi.org/10.1007/978-1-4615-7081-3_13
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