Abstract
Previous work in our laboratory have provided evidence that the membrane glycoprotein M of TGEV is centrally involved in efficient induction of alpha interferon (IFN-α) synthesis by non-immune peripheral blood mononuclear cells incubated with fixed, TGEV-infected cells or inactivated virions. Here we report recent completion of studies initiated to get a better understanding of the nature of the interferogenic determinant(s). Transfected cells expressing TGEV M together with the minor structural component E (formerly called sM) were found to trigger IFN-α synthesis. Co-expression of these two proteins was shown to be necessary and sufficient for assembly and release of pseudoparticles resembling TGEV virions. Purified pseudoparticles exhibited an interferogenic activity close to that of authentic virions. Chimeric recombinant particles expressing BCV M ectodomain also induced IFN. Examination of cell cultures infected by viruses representative of the three Nidovirales genera revealed that the capacity to act as an efficient IFN-α inducer is a common feature of viral particles of the coronavirus genus. Altogether these data bring new insights regarding the putative nature of the viral structure involved in IFN-α induction.
Chapter PDF
Similar content being viewed by others
Keywords
- Equine Arteritis Virus
- Transmissible Gastroenteritis Virus
- Corona Virus
- Equine Dermis
- Porcine Reproductive Respiratory Syndrome Virus
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
References
Baudoux P., Charley B., Laude H., 1995, Recombinant expression of the TGEV membrane protein, in “Corona-and related viruses” (P. Talbot & G. Levy Eds.),
Charley B., Laude H., 1988, Induction of interferon alpha by transmissible gastroenteritis coronavirus : role of transmembrane glycoprotein E1, J. Virol. 62: 8–11.
Fitzgerald-Bocarsly P., 1993, Human Natural Interferon-a producing cells,Pharmacol. Ther. 60: 39–62.
Godet M., L’Haridon R., Vautherot J.F., Laude H., 1992, TGEV coronavirus ORF4 encodes a membrane protein that is incorporated into virions, Virology 188: 666–675.
La Bonnardière C., Laude H., 1981, High interferon titer in newborn pig intestine during experimentally induced viral enteritis, Infec. Immun. 32: 28–31.
Laude H., Chapsal J.M., Gelfi J., Labiau S., Grosclaude J., 1986, Antigenic structure of transmissible gastroenteritis coronavirus I. Properties of monoclonal antibodies directed against virion proteins, J. Gen. Virol. 67: 119–130.
Laude H., Gelfi J., Lavenant L., Charley B., 1992, Single amino acid changes in the viral glycoprotein M affect induction of alpha interferon by coronavirus TGEV, J. Virol. 66: 743–749.
Laude H., Rasschaert D., Huet J.C., 1987, Sequence and N-terminal processing of the transmembrane protein El of the coronavirus transmissible gastroenteritis Virus, J. Gen. Virol. 68: 1687–1693.
Riffault S. Carrat C. Besnardeau L., La Bonnardiere C., Charley B., 1997, In vivo induction of interferon-a in pig by non-infectious coronavirus: tissue localization and in situ phenotypic characterization of interferon-a producing cells, J. Gen. Virol. 78: 2483–2487.
Savoysky E., Boireau P., Finance C., Laporte, J., 1990, Sequence and analysis of BECV Fl5 matrix protein, Res. Virol. 141:411–425.
Vennema H., Godeke G-J., Rossen J.W.A., Vorhout W.F., Horzinek M.C., Opstelten D.-J., Rottier P.J.M., 1996, Nucleocapsid-independent assembly of coronavirus -like particles by co-expression of viral envelope protein genes, EMBO J. 15: 2020–2028.
Woloszyn N., Boireau P., Laporte J., 1990, Nucleotide sequence of the bovine enteric coronavirus Fl5 mRNA 5 and mRNA 6 unique regions, Nucl. Ac. Res. 18: 1303.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1998 Springer Science+Business Media New York
About this chapter
Cite this chapter
Baudoux, P., Besnardeau, L., Carrat, C., Rottier, P., Charley, B., Laude, H. (1998). Interferon Alpha Inducing Property of Coronavirus Particles and Pseudoparticles. In: Enjuanes, L., Siddell, S.G., Spaan, W. (eds) Coronaviruses and Arteriviruses. Advances in Experimental Medicine and Biology, vol 440. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5331-1_49
Download citation
DOI: https://doi.org/10.1007/978-1-4615-5331-1_49
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-7432-9
Online ISBN: 978-1-4615-5331-1
eBook Packages: Springer Book Archive