Abstract
Reduced nicotinamide adenine dinucleotide (NADH) offers one of the main means to transfer energy from the tricarboxylic acid cycle to the respiratory chain in the mitochondria. NADH is situated at the high-energy side of the respiratory chain and during tissue hypoxia accumulates in concentration because less NADH is oxidized to NAD+. The optical properties of NADH and NAD+ clearly differ. The absorption spectrum of a NADH solution shows two maxima at the ultraviolet end of the light spectrum, one at 250 nm and the other at about 340 nm. NAD+, on the other hand has an absorption maximum at 250 nm and almost does not absorb light above 300 nm [Renault et al. 1982]. Upon excitation with UV-light NADH, unlike NAD+, fluoresces in the blue (broad-band emission centered around 460 nm). Chance and co-workers pioneered this fluorescence property of NADH as an indicator of the intramitochondrial redox state and, in the presence of sufficient substrate and phosphates, as an indicator of cellular oxygen requirements [Chance et al. 1962, 1976]. In order to gain insight into the metabolic properties of biological material from various sources, NADH fluorescence measurements have now been applied to single cells, tissue-slices and intact organs (both saline-perfused and in vivo). As absorption and scatter of light by chromophores and corpuscles in tissue affect measurement of NADH fluorescence, several compensation methods have been introduced. Especially when studying NADH fluorescence in vivo, the effect of blood has to be taken into consideration for correct interpretation of the fluorescence measurements. The purpose of this brief review is to summarize the work that has been done on the application of NADH fluorescence measurements to the study of the metabolic state of tissue in vivo.
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Ince, C., Coremans, J.M.C.C., Bruining, H.A. (1992). In Vivo NADH Fluorescence. In: Erdmann, W., Bruley, D.F. (eds) Oxygen Transport to Tissue XIV. Advances in Experimental Medicine and Biology, vol 317. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3428-0_30
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