Abstract
Magnetic resonance imaging (MRI) has become an important technique for noninvasive cell tracking in preclinical research. Following appropriate cell labeling MRI can be used to detect larger cell cohorts and also single cells in vivo in mice. Cell distribution to different organs such as brain, liver, spleen, and kidneys can be visualized, semi-quantified, and followed over time. Thus, the fate of single tumor cells and their eventual development to solid metastases could be investigated. Mesenchymal stromal cells can be used as a paradigm for metastasizing tumor cells. We have demonstrated a strategy for magnetic and fluorescent co-labeling of mesenchymal stromal cells (MSC), ultrasound-guided intracardial cell injection with efficient systemic cell delivery, and high-resolution MRI for repetitive visualization of disseminated co-labeled MSC on a single-cell level in vivo in mice. Furthermore, the fluorescent labeling of cells enabled effective histopathological validation.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Anderson SA, Shukaliak-Quandt J, Jordan EK et al (2004) Magnetic resonance imaging of labeled T-cells in a mouse model of multiple sclerosis. Ann Neurol 55(5):654–659
Shapiro EM, Gonzalez-Perez O, Manuel García-Verdugo J, Alvarez-Buylla A, Koretsky AP (2006) Magnetic resonance imaging of the migration of neuronal precursors generated in the adult rodent brain. Neuroimage 32(3):1150–1157
Arbab AS, Pandit SD, Anderson SA et al (2006) Magnetic resonance imaging and confocal microscopy studies of magnetically labeled endothelial progenitor cells trafficking to sites of tumor angiogenesis. Stem Cells 24(3):671–678
Bos C, Delmas Y, Desmoulière A et al (2004) In vivo MR imaging of intravascularly injected magnetically labeled mesenchymal stem cells in rat kidney and liver. Radiology 233(3):781–789
Heyn C, Ronald JA, Ramadan SS et al (2006) In vivo MRI of cancer cell fate at the single-cell level in a mouse model of breast cancer metastasis to the brain. Magn Reson Med 56(5):1001–1010
Shapiro EM, Skrtic S, Koretsky AP (2005) Sizing it up: cellular MRI using micron-sized iron oxide particles. Magn Reson Med 53(2):329–338
Fleige G, Seeberger F, Laux D et al (2002) In vitro characterization of two different ultrasmall iron oxide particles for magnetic resonance cell tracking. Invest Radiol 37(9):482–488
Frank JA, Zywicke H, Jordan EK et al (2002) Magnetic intracellular labeling of mammalian cells by combining (FDA-approved) superparamagnetic iron oxide MR contrast agents and commonly used transfection agents. Acad Radiol 9(Suppl 2):S484–S487
Sun R, Dittrich J, Le-Huu M et al (2005) Physical and biological characterization of superparamagnetic iron oxide- and ultrasmall superparamagnetic iron oxide-labeled cells: a comparison. Invest Radiol 40(8):504–513
Heyn C, Ronald JA, Mackenzie LT et al (2006) In vivo magnetic resonance imaging of single cells in mouse brain with optical validation. Magn Reson Med 55(1):23–29
Ittrich H, Lange C, Tögel F et al (2007) In vivo magnetic resonance imaging of iron oxide-labeled, arterially-injected mesenchymal stem cells in kidneys of rats with acute ischemic kidney injury: detection and monitoring at 3T. J Magn Reson Imaging 25(6):1179–1191
Mamani JB, Malheiros JM, Cardoso EF, Tannús A, Silveira PH, Gamarra LF (2012) In vivo magnetic resonance imaging tracking of C6 glioma cells labeled with superparamagnetic iron oxide nanoparticles. Einstein (Sao Paulo) 10(2):164–170
Schrepfer S, Deuse T, Reichenspurner H, Fischbein MP, Robbins RC, Pelletier MP (2007) Stem cell transplantation: the lung barrier. Transplant Proc 39(2):573–576
Springer ML, Sievers RE, Viswanathan MN et al (2005) Closed-chest cell injections into mouse myocardium guided by high-resolution echocardiography. Am J Physiol Heart Circ Physiol 289(3):H1307–H1314
Acknowledgements
The authors would like to thank Professor Udo Schumacher, Dr. Claudia Lange, and Dr. Daniel Wicklein for providing MSC, technical support, and helpful discussions. Dr. Michael Didié is granted for his support with the small-animal ultrasound system.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2014 Springer Science+Business Media, New York
About this protocol
Cite this protocol
Salamon, J., Peldschus, K. (2014). Ultrasound-Guided Intracardial Injection and In Vivo Magnetic Resonance Imaging of Single Cells in Mice as a Paradigm for Hematogenous Metastases. In: Dwek, M., Schumacher, U., Brooks, S. (eds) Metastasis Research Protocols. Methods in Molecular Biology, vol 1070. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4614-8244-4_15
Download citation
DOI: https://doi.org/10.1007/978-1-4614-8244-4_15
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4614-8243-7
Online ISBN: 978-1-4614-8244-4
eBook Packages: Springer Protocols