Abstract
The use of DNA probes is now becoming established as one means for the identification of insect vectors of disease particularly where more traditional methods are not available1,2,3. Currently, their use involves the application of either a portion of the fly specimen or purified DNA from the specimen onto nitrocellulose. The DNA in the sample is then denatured with alkali and immobilised by baking at 80°C for 2 hours. Subsequently, a radioactive DNA probe is hybridised to the nitrocellulose and identification results from inspection of the autoradiographic signal obtained.
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© 1989 Springer Science+Business Media New York
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Crampton, J., Knapp, T., Ward, R. (1989). DNA Probes for Vector Taxonomy. In: Hart, D.T. (eds) Leishmaniasis. NATO ASI Series, vol 171. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-1575-9_122
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DOI: https://doi.org/10.1007/978-1-4613-1575-9_122
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