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Saponins from Medicago SPP.: Chemical Characterization and Biological Activity Against Insects

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Saponins Used in Food and Agriculture

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 405))

Abstract

The occurrence and distribution of secondary metabolites in plants, from an ecological viewpoint, can be associated with a defensive strategy of plants against herbivores. In order to limit damage from microbes, insects, or vertebrate predation, plants have developed complex metabolic pathways to produce specific secondary compounds that are associated with defense against pests and pathogens. Complex antibiotic substances such as alkaloids and terpenes, as well as proteins such as protease inhibitors, provide inducible or constitutive defense both in woody and in herbaceous plants.

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References

  • Abbott, W., 1925, A method of computing the effectivenes of an insecticide, J. Econ. Entomol. 18:265.

    CAS  Google Scholar 

  • Ameenuddin, S., Bird, H.R., Pringle, DJ., and Sunde, M.L., 1983, Studies on the utilization of leaf protein concentrates as a protein source in poultry nutrition, Poultry Sci. 62:505.

    CAS  Google Scholar 

  • Applebaum, S.W., and Birk, Y., 1979 In: Herbivores — Their Interaction with Secondary Plant Metabolites; Rosenthal, G.A. and Janzen, D.H., Eds., Academic Press, New York.

    Google Scholar 

  • Applebaum, S.W., Marco, S., and Birk, Y., 1969, Saponins as a possible factor of resistance of legume seeds to attack of insects, J. Agric. Food Chem. 17:618.

    Article  CAS  Google Scholar 

  • Bocsa, I., 1990, Evaluation of the breeding results on low saponin content in lucerne, Proc. IXEucarpia Medicago sativa Group Meeting, Szarvas, Hungary, p. 37.

    Google Scholar 

  • Brawn, P.R., Lindner, N.M., Miller, J.M. and Telling, G.M., 1981, A gas chromatographic method for the determination of medicagenic acid in lucerne (alfalfa) leaf protein concentrate, J. Sei. Food Agric. 32:1157.

    Article  CAS  Google Scholar 

  • Budzikiewicz, H., Wilson, J.M. and Djerassi, C, 1963, Mass spectrometry in structural and stereochemical problems. XXXII. Pentacyclic triterpenes, J. Am. Chem. Soc. 85:3688.

    Article  CAS  Google Scholar 

  • Cheeke, P.R., 1971, Nutritional and physiological implications of saponins: a review, Can. J. Anim. Sei. 51:621.

    Article  CAS  Google Scholar 

  • Cheeke, P.R., 1983, Biological properties and nutritional significance of legume saponins. In: Leaf Protein Concentrate, Telek L. & Graham H., Eds., AVI Publishing, Westport, CT, USA.

    Google Scholar 

  • Cheeke, RR., Kinzell, J.H., and Pedersen, M.W., 1977, Influence of saponins on alfalfa utilization by rats, rabbit and swine, /. Anim. Sei. 45:476.

    CAS  Google Scholar 

  • Fenwick, G.R., Price, K.R., Tsukamoto, C, and Okubo, K., 1991, Saponins. In: Toxicant Substances in Crop Plants, Felix D’Mello, J.P., Duffus, CM. & Duffus, J.H., Eds., Royal Society of Chemistry, London, UK.

    Google Scholar 

  • Gee, J.M., Price, K.R., Ridout, C.L., Johnson, I.T., and Fenwick, G.R., 1989, The effect of some purified saponins on transmural p.d. in mammalian small intestine, Toxicol, in vitro 3:85.

    Article  CAS  Google Scholar 

  • Jenkins, K.J., and Atwal, A.S., 1994, Effects of dietary saponins on fecal bile acids and neutral sterols, and availability of vitamin A and E in the chick, J. Nutr. Biochem. Newton 5:134.

    Article  CAS  Google Scholar 

  • Jurzysta, M., 1979, Haemolytic micromethod for rapid estimation of toxic alfalfa saponin, Acta Agrobot. 32:5.

    CAS  Google Scholar 

  • Jurzysta, M., 1984, Transformation of soyasapogenol B into soyasapogenols C, D and F under acidic conditions, Proc. 14th Int. Symp. Natural Products, p. 127.

    Google Scholar 

  • Jurzysta, M. and Jurzysta, A., 1978, Gas—liquid chromatography of trimethylsilyl ethers of soyasapogenols and medicagenic acid, J. Chromatogr. 148:517.

    Article  CAS  Google Scholar 

  • Jurzysta, M., and Nowacki, E., 1979, Saponins of the genus Medicago, Acta Agrobot. 32:13.

    CAS  Google Scholar 

  • Luc, CD., and Jorgensen, N.A., 1987, Alfalfa saponins affect site and extent of nutrient digestion in ruminants, J. Nutr. 117:919.

    Google Scholar 

  • Luc, CD., Tsai, L.S., Schaefer, D.M. and Jorgensen, N.A., 1987, Alteration of fermentation in continuous culture of mixed rumen bacteria by isolated alfalfa saponins, Dairy Sci. 70:799.

    Article  Google Scholar 

  • Malinow, M.R., 1984, Saponins and cholesterol metabolism, Atherosclerosis 50:117.

    Article  PubMed  CAS  Google Scholar 

  • Massiot, G., Lavaud, C, Besson, V., Le Men-Olivier, L. and Binst, G., 1991, Saponins from aerial parts of alfalfa (Medicago sativa), J. Agric. Food Chem. 39:78.

    Article  CAS  Google Scholar 

  • Nowacka, J., and Oleszek, W, 1994, Determination of alfalfa (Medicago sativa) saponins by high—performance liquid chromatography, J. Agric. Food Chem. 42:727.

    Article  CAS  Google Scholar 

  • Oakenfull, D. and Sidhu, G.S., 1989, Saponins, In: Toxicants of Plant Origin, Cheeke, RR., Ed., CRC Press, Inc., Boca Raton, Florida.

    Google Scholar 

  • Odoardi, M., Santelli, G., Cremona, R., Berardo. N., Stringi, L. and Giambalvo, D., 1994, Screening Atriplex halimus and Medicago arborea populations for saponin content, Proc. Eucarpia Fodder Crop Section Meeting, Brugge, Belgium, p. 103.

    Google Scholar 

  • Oleszek, W., 1988, Solid phase extraction-fractionation of alfalfa saponins, J. Sei. Food Agric. 44:43.

    Article  CAS  Google Scholar 

  • Oleszek, W., and Jurzysta, M., 1986, Isolation, chemical characterization and biological activity of alfalfa (Medicago media Pers.) root saponins, Acta Soc. Bot. Pol. 55:23.

    CAS  Google Scholar 

  • Oleszek, W., Jurzysta, M., Ploszynski, M., Colquhoun, I.J., Price, K.R., and Fenwick, G.R., 1992, Zanhic acid tridesmoside and other dominant saponins from alfalfa (Medicago sativa L.) aerial parts, J. Agric. Food Chem. 40:191.

    Article  CAS  Google Scholar 

  • Oleszek, W., Jurzysta, M., Price, K.R., and Fenwick, G.R., 1990a, High performance liquid chromatography of alfalfa root saponins, J. Chromatogr. 519:109.

    Article  CAS  Google Scholar 

  • Oleszek, W., Price, K.R., Colquhoun, I.J., Jurzysta, M., Ploszynski, M., and Fenwick, G.R., 1990b, Isolation and identification of alfalfa (Medicago sativa) root saponins: their activity in relation to a fungal bioassay, J. Agric. Food Chem. 38:1810.

    Article  CAS  Google Scholar 

  • Pedersen, M.W., Berrang, B., Wall, M.E., and Davis, K.H., Jr., 1973, Modification of saponin characteristics of alfalfa by selection, Crop Sci. 13:731.

    Article  CAS  Google Scholar 

  • Pedersen, M.W., and Wang, L.C., 1971, Modification of saponin content of alfalfa through selection, Crop Sci. 11:833.

    Article  Google Scholar 

  • Piano, E., Berardo, N., Pecetti, L., Valentini, P., and Odoardi, M., 1994, Some observations on the qualitative characteristics of grazing-type lucerne, Proc. Eucarpia Fodder Crop Section Meeting, Brugge, Belgium, p. 95.

    Google Scholar 

  • Pracros, P., 1988, Mesure de l’activité des saponins de la lucerne par les larves du ver de la farine: Tenebrio molitor L. (Coléoptère, Tenebrionidae). I. — Comparison avec les résultats de divers test biologiques, Agronomie 8:257.

    Article  Google Scholar 

  • Price, K.R., Fenwick, G.R. and Jurzysta, M., 1986, Soyasapogenols — separation, analysis and interconversions, J. Sei. Food Agric. 37:1027.

    Article  CAS  Google Scholar 

  • Price, K.R., Johnson, I.T., and Fenwick, G.R., 1987, The chemistry and biological significance of saponins in food and feedingstuffs, CRC Crit. Rev. Food Sei. Nutr. 26:27.

    Article  CAS  Google Scholar 

  • Rao, D. and Boris, G., 1987, Simple gas chromatographic method for the determination of medicagenic acid in alfalfa (Medicago sativa), J. Chromatogr. 410:169.

    Article  PubMed  CAS  Google Scholar 

  • Rotili, P., and Zannone, L., 1981, Alfalfa breeding for forage yield and low saponin content. Proc. XIV Int. Grassl. Congress, Lexington, KY, USA, p. 229.

    Google Scholar 

  • Sidhu, G.S., and Oakenfull, D.G., 1986, A mechanism for the hypocholesterolaemic activity of saponins, Br. J. Nutr. 55:643.

    Article  PubMed  CAS  Google Scholar 

  • Tava, A., Forti, D., and Odoardi, M., 1992, Alfalfa saponins: isolation, chemical characterization and biological activity against insects, Proc. XEucarpia Medicago spp Group Meeting, Lodi, Italy, p. 283.

    Google Scholar 

  • Tava, A., Oleszek, W, Jurzysta, M., Berardo, N., and Odoardi, M., 1993, Alfalfa saponins: isolation and quantification in two different cultivars, Phytochem. Anal. 4:269.

    Article  CAS  Google Scholar 

  • West, L.G., 1979, Identification of oleanolic acid from saponins present in lucerne (alfalfa) roots, J. Sci. Food Agric. 30:540.

    Article  CAS  Google Scholar 

  • Zimmer, D.E., Pedersen, M.W., and McGuire, C.F., 1967, A bioassay for alfalfa saponins using the fungus Trichoderma viride Pers., Crop Sci. 1:223.

    Article  Google Scholar 

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© 1996 Plenum Press, New York

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Tava, A., Odoardi, M. (1996). Saponins from Medicago SPP.: Chemical Characterization and Biological Activity Against Insects. In: Waller, G.R., Yamasaki, K. (eds) Saponins Used in Food and Agriculture. Advances in Experimental Medicine and Biology, vol 405. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-0413-5_9

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  • DOI: https://doi.org/10.1007/978-1-4613-0413-5_9

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-8041-2

  • Online ISBN: 978-1-4613-0413-5

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