Abstract
This procedure is sometimes used with recalcitrant species or older plant tissues. Because the procedure employs a phenol:chloroform extraction it is slightly more time-consuming than the method described in the previous chapter and generates organic wastes. However, the recovered DNA usually digests well with restriction enzymes and stores for long periods without signs of degradation.
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See notes 1–5 in the Preceding Chapter.
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© 1994 Springer-Verlag New York, Inc.
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Chen, J., Dellaporta, S. (1994). Urea-based Plant DNA Miniprep. In: Freeling, M., Walbot, V. (eds) The Maize Handbook. Springer Lab Manuals. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-2694-9_85
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DOI: https://doi.org/10.1007/978-1-4612-2694-9_85
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