Abstract
In order to remove or reduce the concentrations of toxic substances present in the sugarcane bagasse hemicellulose hydrolysate for xylose-to-xylitol bioconversion, the hydrolysate was pretreated by changing the initial pH level through the combination of different bases and acids with or without the subsequent addition of activated charcoal. Attention was given to the influence of the fermentation time as well.
The experiments were based on multivariate statistical concepts, with the application of fractional factorial design techniques to identify the important variables in the process. Subsequently, the levels of these variables were quantified by the response surface methodology, which permitted the establishment of the best pretreatment procedure with a xylose-to-xylitol bioconversion efficiency of 86.2%. This procedure consisted in increasing the pH of the hydrolysate from 0.5 to 7.0 with CaO and reducing it to 5.5 with H3PO4. Next, the hydrolysate was treated with activated charcoal (2.4%). The highest xylitol yield (0.79 g/g) corresponded to a productivity of 0.47 g/L/h.
Author to whom all correspondence and reprint requests should be addressed.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
References
Felipe, M. G. A., Vitolo, M., and Mancilha, I. M. (1996A), Acta Biotechnologica 16, 73–79.
Felipe, M. G. A., Alves, L. A., Silva, S. S., Roberto, I. C, Mancilha, I. M., and Almeida e Silva, J. B., (1996B) Bioresource Technol. 56, 281–283.
Melaja, A. J. and Hämäläinen, L. (1997), Process for Making Xylitol. US Patent no. 4.008.285. 1975, 15 fev.
Domingues, J. M., Cheng, S. G., and Tsao, G. T. (1996), Applied Biochem. Biotechnol. 57/58, 49–56.
Meyrial, V., Delgenes, J. P. Molletta, R., and Navarro, J. M. (1991), Biotechnol. Lett. 13, 281–286.
Nolleau, V., Preziosi-Belloy, L., Delgenes, J. P., and Delgenes, J. M. (1993), Curr. Microbiol. 27, 191–197.
Felipe, M. G. A., Vitolo, Mancilha, I. M., and Silva, S. S. (1997), J. Indust. Microbiol. 18, 251–254.
Roberto, I. C, Felipe, M. G. A., Lacis, L. S., Silva, S. S., and Mancilha, L M. (1991), Bioresource Technol. 36, 271–275.
Barbosa, M. F. S., Medeiros, M. B., Mancilha, I. M., Schneider, H., and Lee, H. (1988), J. Indust. Microbiol. 3, 241–251.
Felipe, M. G. A., Vieira, D. C, Vitolo. M., Silva, S. S., Roberto, I. C., Mancilha, I. M., and Rosa, S. A. M. (1995), J. Basic Microbiol. 35, 171–177.
Box, G. E. P., Hunter, W. G., and Hunter, J. S. (1978), Statistics for Experimenters: An Introduction to Design, Data Analysis and Model Building. Wiley, New York.
Barros Neto, B., Scarminio, I. S., and Bruns, R. E. (1995), Planejamento e Otimizaçao de Experimentes, 1 ed., Editora da UNICAMP, Campinas.
Chen, L. F. and Gong, C. S. (1985), J. Food Sci. 50, 226–228.
Felipe, M. G. A., Mancilha, I. M., Vitolo, M., Roberto, I. C., Silva, S. S., and Rosa, S. A. M. (1993), Arquivos de Biologia e Technologia, 36, 103–114.
Roberto I. C., Mancilha, I. M., Souza, C. M. A., Felipe, M. G. A., Sato, S., and Castro, H. F. (1994), Biotechnol. Lett. 16, 1211–1216.
van Zyl, C., Prior, B. A., and Du Preez, J. C. (1988), Appl. Biochem. Biotechnol. 17, 357–369.
Parajó, J. C., Domingues, H., and Domingues, J. M. (1996B) Bioresource Technol. 57, 179–185.
Parajó, J. C, Domingues, H., and Domingues, J. M. (1996A), Biotechnol. Lett. 18, 593–598.
Chung, I. S., and Lee, Y. Y. (1985), Biotechnol Bioengineer. 27, 308–315.
Wang, R. C., Kuo, C. C, and Shyu, C. C. (1997), J. Chem. Tech. Biotechnol. 68, 187–194.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1998 Springer Science+Business Media New York
About this chapter
Cite this chapter
Alves, L.A., Felipe, M.G.A., Silva, J.B.A.E., Silva, S.S., Prata, A.M.R. (1998). Pretreatment of Sugarcane Bagasse Hemicellulose Hydrolysate for Xylitol Production by Candida guilliermondii. In: Finkelstein, M., Davison, B.H. (eds) Biotechnology for Fuels and Chemicals. Applied Biochemistry and Biotechnology. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4612-1814-2_9
Download citation
DOI: https://doi.org/10.1007/978-1-4612-1814-2_9
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-4612-7295-3
Online ISBN: 978-1-4612-1814-2
eBook Packages: Springer Book Archive