Abstract
In recent years biosurfactants have attracted attention because of their low toxicity, biodegradability and ecological acceptability. However, their use is currently extremely limited due to their high cost in relation to that of chemical surfactants. Solid-state cultivation represents an alternative technology for biosurfactant production that can bring two important advantages: firstly, it allows the use of inexpensive substrates and, secondly, it avoids the problem of foaming that complicates submerged cultivation processes for biosurfactant production. In this chapter we show that, despite its potential, to date relatively little attention has been given to solid-state cultivation for biosurfactant production. We also note that this cultivation technique brings its own challenges, such as the selection of a bioreactor type that will allow adequate heat removal, of substrates with appropriate physico-chemical properties and of methods for monitoring of the cultivation process and recovering the biosurfactants from the fermented solid. With suitable efforts in research, solid-state cultivation can be used for large-scale production of biosurfactants.
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References
Karanth NGK, Deo PG, Veenanadig NK. Microbial production of biosurfactants and their Importance. Curr Sci 1999; 77:116–126.
Makkar RS, Cameotra SS. An update on the use of unconventional substrates for biosurfactant production and their new applications. App Microbiol Biotechnol 2002; 58:428–434.
Nitschke M, Costa SGVAO, Contiero J. Rhamnolipid surfactants: Na update on the general aspects of these remarkable biomolecules. Biotechnol Progr 2005; 21:1593–1600.
Nitschke M, Costa SGVAO, Haddad R et al. Oil waste as unconventional substrate for rhamnolipid biosurfactant production by Pseudomonas aeruginosa LBI. Biotechnol Progr 2005; 21:1562–1566.
Costa SGVAO, Nitschke M, Haddad R et al. Production of Pseudomonas aeruginosa LBI rhamnolipids following growth on Brazilian native oils. Process Biochem 2006; 41:483–488.
Lang S. Biological amphiphiles (microbial biosurfactants). Curr Opin Colloid Interface Sci 2002; 7:12–20.
Batista SB. Bactérias de ambientes contaminados com petróleo ou derivados produtoras de surfactantes e emulsificantes. Dissertation, Universidade Federal de Viçosa 2002; 1–32.
Kosaric N, Cairns WL, Gray NCC. Introduction: Biotechnology and the surfactant industry. In: Kosaric N, Cairns WL, eds. Biosurfactants and Biotechnology. New York: M Dekker, 1987:163–181.
Mukherjee S, Das P, Sen R. Towards commercial production of microbial surfactants. Trends Biotechnol 2006; 24:509–515.
Zajic JE, Seffens W. Biosurfactants. Crit Revi Biotechnol 1984; 1:87–107.
Jacobucci DFC. Estudo da influência de biosurfactantes na biorremediação de efluentes oleosos. Dissertation, Universidade Estadual de Campinas 2000;1–117.
Veenanadig NK, Gowthaman MK, Karanth NGK. Scale up studies for the production of biosurfactant in packed column bioreactor. Bioprocess Eng 2000; 22:95–99.
Lee BS, Kim EK. Lipopeptide production from Bacillus sp. GB16 using a novel oxygenation method. Enzyme Microb Technol 2004; 35:639–647.
Yeh MS, Wei TH, Chang JS. Bioreactor design for enhanced carrier-assisted surfactin production with Bacillus subtilis. Process Biochem 2006; 41:1799–1805.
Deshpande NS, Barigou M. Performance characteristics of novel mechanical foam breakers in a stirred tank reactor. J Chem Tech Biotechnol 1999; 74:979–987.
Vadar-Sukan F. Foaming: Consequences, prevention and destruction. Biotechnol Adv 1998; 16:913–948.
Mitchell DA, Krieger N, Berovic M. Solid-state fermentation bioreactors: Fundamentals of design and operation. Heidelberg: Springer 2006:1–450.
Ohno A, Ano T, Shoda M. Production of antifungal antibiotic, iturin in a solid state fermentation by Bacillus subtilis NB22 using wheat bran as a substrate. Biotechnol Lett 1992; 14:817–822.
Ohno A, Ano T, Shoda M. Production of the antifungal peptide antibiotic, iturin by Bacillus subtilis NB22 in solid state fermentation. J Ferment Bioeng 1993; 75:23–27.
Ohno A, Ano T, Shoda M. Production of a Lipopeptide Antibiotic, Surfactin, by Recombinant Bacillus subtilis in solid state fermentation. Biotechnol Bioeng 1995; 47:209–214.
Ohno A, Ano T, Shoda M. Effect of temperature on production of lipopeptide antibiotics, iturin A and surfactin by a dual producer, Bacillus subtilis RB14, in solid-stage fermentation. J Ferment Bioeng 1995; 80:517–519.
Ohno A, Ano T, Shoda M. Use of soybean curd residue, okara, for the solid state substrate in the production of a lipopeptide antibiotic, Iturin A, by Bacillus subtilis NB22 Process Biochem 1996; 31:801–806.
Das K, Mukherjee AK. Comparison of lipopeptide biosurfactants production by bacillus subtilis strains in submerged and solid state fermentation systems using a cheap carbon some industrial applications of biosurfactants. Process Biochem 2007; 42:1191–1199.
Krieger N, Mitchell DA, Camilios Neto D et al. Inventors; Universidade federal do paraná (UFPR), assignee. Produção de ramnolipídeos por fermentação em estado sólido. Patent 2007:PI0701366-3.
Meira JA. Produção de biosurfactantes por fermentação no estado sólido e desenvolvimento de aplicações para tratamento de solos contaminados por hidrocarbonetos. Dissertation, Universidade Federal do Paraná 2007;1-94.
Martins VG, Kalil SJ, Bertolin TE et al. Solid state biosurfactant production in a fixed-bed column bioreactor. Z Naturforsch C 2006; 61:721–726.
Lonsane BK, Kriahnaiah MM. Product leaching and downstream processing. In: Doelle HW, Mitchell DA, Rolz CE, eds. Solid State Cultivation. London: Elsevier, 1992:147–171.
Kristensen JB, Börjesson J, Bruun MH et al. Use of surface active additives in enzymatic hydrolysis of wheat straw lignocelluloses. Enzyme Microb Technol 2007; 40:888–895.
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Krieger, N., Neto, D.C., Mitchell, D.A. (2010). Production of Microbial Biosurfactants by Solid-State Cultivation. In: Sen, R. (eds) Biosurfactants. Advances in Experimental Medicine and Biology, vol 672. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-5979-9_15
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DOI: https://doi.org/10.1007/978-1-4419-5979-9_15
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