Abstract
Epigenetic modification are heritable changes in gene expression not encoded by the DNA sequence therefore playing a significant role in a broad range of biological processes and diseases.
A key player of the epigenetic modifications is the DNA methylation, a process involving the covalent transfer of a methyl group to the C-5 position of the cytosine ring of DNA forming 5-methylcytosine (5mC), catalyzed by DNA methyltransferases. Altering the structure of the chromatin, DNA methylation has the potential to down-regulate gene expression.
The here presented protocol shows a method to obtain DNA samples ready for NGS sequencing for genome-wide methylation analysis.
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Mancia, A. (2022). Genome-Wide DNA Methylation Protocol for Epigenetics Studies. In: Verde, C., Giordano, D. (eds) Marine Genomics. Methods in Molecular Biology, vol 2498. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2313-8_2
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DOI: https://doi.org/10.1007/978-1-0716-2313-8_2
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