Abstract
The development of new biocatalysts as synthetic tools for chemists has been expanding rapidly over the last several years. It is now possible either to discover or engineer enzymes with unique substrate specificities and selectivities that are stable and robust for organic synthesis applications. This has been made possible by the application of the newest screening and selection technologies that allow rapid identification of enzyme activities from diverse sources.We focus on how to recognize and tackle important issues during the strategic design and implementation of screening for novel enzymes. We also review the approaches available for biocatalyst discovery and relate them to the isolation of thermostable enantioselective esterases and alcohol dehydrogenases for the purpose of illustration and discussion.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
References
Schoffers E, Golebiowski A, Johnson CR (1996) Tetrahedron 52:3769
Crabb WD, Mitchinson C (1997) Trends Biotechnol 15:349
Gerhartz W (1990) Enzymes in industry. VCH, Weinheim
Wong C-H, Whitesides GM (1994) Enzymes in synthetic organic chemistry. Pergamon, Oxford
Santaniello E, Ferraboschi P, Grisenti P, Manzocchi A (1992) Chem Rev 92:1071
Zaks A, Dodds DR (1997) Drug Discovery Today 2:513
Shimizu S, Ogawa J, Kataoka M, Kobayashi M (1997) Adv Biochem Eng Biotechnol 58:45
Turner MK (1995) Trends Biotechnol 13:173
Schwardt E (1990) Food Biotechnol 4:337
Roberts S (1997) Preparative biotransformations. Wiley, Chichester
Arnold FH, Moore JC (1997) Adv Biochem Eng Biotechnol 58:1
Owusu RK, Cowan DA (1990) Enzyme Microb Technol 12:374
Liao HH (1993) Enzyme Microb Technol 15:286
Tosa T, Shibatani T (1995) Ann N Y Acad Sci 750:364
Mazid MA (1993) Bio-Technology 11:690
St Clair NL, Navia MA (1992) J Am Chem Soc 114:7314
Kuchner O, Arnold FH (1997) Trends Biotechnol 15:523
Annual Product Guide Issue (1998) Nature Biotechnol 15:1107
Short JM (1997) Nature Biotechnol 15:1322
Lalonde J (1997) Chemtech 27:38
Dalboge H, Lange L (1998) Trends Biotechnol 16:265
Hummel W (1997) Exs 80:49
Chartrain M, Armstrong J, Katz L, King S, Reddy J, Shi YJ, Tschaen D, Greasham R (1996) Ann N Y Acad Sci 799:612
(1984) ATCC media handbook. American Type Culture Collection, Rockville, MD
Bergey DH, Holt JG (1993) Bergey’s manual of determinative bacteriology. Williams & Wilkins, Baltimore
Giovannoni SJ, DeLong EF, Olsen GJ, Pace NR (1988) J Bacteriol 170:720
Busse HJ, Denner EBM, Lubitz W (1996) J Biotechnol 47:3
Sambrook J, Maniatis T, Fritsch EF (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
Amann R, Ludwig W, Schliefer K-H (1995) Microbiol Rev 59:143
Fastrez J (1997) Mol Biotechnol 7:37
Vonstein V, Johnson SP, Yu H, Casadaban MJ, Pagratis NC, Weber JM, Demirjian DC (1995) J Bacteriol 177:4540
LaRossa RA (1996) In: Neidhart FC, Curtiss III R, Ingraham JL, Lin ECC, Low KB, Magasanik B, Reznikoff WS, Riley M, Schaechter M, Umbarger HE (eds) Escherichia coli and Salmonella, vol 2. ASM Press, Washington, DC, p 2527
Michal G, Möllering H, Siedel J (1983) In: Bergmeyer HU (ed) Methods of enzymatic analysis, vol I. Verlag Chemie, Weinheim p 197
Alvarez AM, Ibanez M, Rotger R (1993) Biotechniques 15:974
Houston JG, Banks M (1997) Curr Opin Biotechnol 8:734
Cheng J, Sheldon EL, Wu L, Uribe A, Gerrue LO, Carrino J, Heller MJ, Oconnell JP (1998) Nature Biotechnol 16:541
Hadd AG, Raymond DE, Halliwell JW, Jacobson SC, Ramsey JM (1997) Anal Chem 69:3407
Jones P, Watson A, Davies M, Stubbings S (1992) Nucl Acids Res 20:4599
Sheppard PO, Grant FJ, Oort PJ, Sprecher CA, Foster DC, Hagen FS, Upshall A, McKnight GL, O’Hara PJ (1994) Gene 150:163
Madden TL, Tatusov RL, Zhang J (1996) Methods Enzymol 266:131
Koonin EV, Tatusov RL, Rudd KE (1996) Methods Enzymol 266:295
Tatusov RL, Koonin EV, Lipman DJ (1997) Science 278:631
Gaasterland T, Sensen CW (1996) Biochimie 78:302
Dsouza M, Larsen N, Overbeek R (1997) Trends Genet 13:497
Govardhan CP, Margolin AL (1995) Chem Ind 17:689
Weber JM, Johnson SP, Vonstein V, Casadaban MJ, Demirjian DC (1995) Biotechnology (NY) 13:271
Casadaban MJ, Martinez-Arias A, Shapira SK, Chou J (1983) Methods Enzymol 100:293
Casadaban MJ, Chou J, Cohen SN (1980) J Bacteriol 143:971
Miller JH (1991) A short course in bacterial genetics: a laboratory manual and handbook for Escherichia coli and related bacteria. Cold Spring Harbor Laboratories Press, Cold Spring Harbor
Kwaan HC, Friedman RB, Szczecinski M (1978) Thromb Res 13:5
Hron WT, Menahan LA (1981) J Lipid Res 22:377
Bowyer DE, Cridland JS, King JP (1979) J Lipid Res 19:274
Ulitzer S, Heller M (1981) Methods Enzymol 72:338
Bjokhem I, Arner P, Thore A, Ostman J (1981) J Lipid Res 22:1142
Mizuno K, Toyosato M, Yabumoto S, Tauimizu I, Hirakawa H (1980) Anal Biochem 108:6
Sowerby JM, Ottaway JH (1961) Biochem J 79:21P
Conway T, Sewell GW, Osman YA, Ingram LO (1987) J Bacteriol 169:2591
Skursky L, Kovár J, Stachova M (1979) Anal Biochem 99:65
Quiros M, Sanchez VM, Brieva R, Rebolledo F, Gotor V (1993) Tetrahedron Asymmetry 4:1105
Gotor V, Brieva R, González C, Rebolledo F (1991) Tetrahedron 47:9207
Chen C-H, Fujimoto Y, Girdaukas G, Sih CJ (1982) J Am Chem Soc 104:104
Stecher H, Faber K (1997) Synthesis 1
Janes L, Löwendhal C, Kazlauskas R (1998) Eur J Chem (1998) 4:2317
Whittaker RG, Manthey MK, LeBrocque DS, Hayes PJ (1994) Anal Biochem 220:238
Bornscheuer UT, Enzelberger MM, Altenbuchner J, Meyer HH (1998) Stratagene Tech Bull 11:16
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1999 Springer Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Demirjian, D.C., Shah, P.C., Morís-Varas, F. (1999). Screening for Novel Enzymes. In: Fessner, WD., et al. Biocatalysis - From Discovery to Application. Topics in Current Chemistry, vol 200. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3-540-68116-7_1
Download citation
DOI: https://doi.org/10.1007/3-540-68116-7_1
Published:
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-64942-7
Online ISBN: 978-3-540-68116-8
eBook Packages: Springer Book Archive