Skip to main content
SpringerLink
Log in

Metabolic Engineering of the Morphology of Aspergillus

  • Chapter
  • First Online:
Metabolic Engineering

Part of the book series: Advances in Biochemical Engineering/Biotechnology ((ABE,volume 73))

Abstract

The morphology of filamentous organisms in submerged cultivation is a subject of considerable interest, notably due to the influence of morphology on process productivity. The relationship between process parameters and morphology is complex: the interactions between process variables, productivity, rheology, and macro- and micro-morphology create difficulties in defining and separating cause and effect. Additionally, organism physiology contributes a further level of complexity which means that the desired morphology (for optimum process performance and productivity) is likely to be process specific. However, a number of studies with increasingly powerful image analysis systems have yielded valuable information on what these desirable morphologies are likely to be. In parallel, studies on a variety of morphological mutants means that information on the genes involved in morphology is beginning to emerge. Indeed, we are now beginning to understand how morphology may be controlled at the molecular level. Coupling this knowledge with the tools of molecular biology means that it is now possible to design and engineer the morphology of organisms for specific bioprocesses. Tailor making strains with defined morphologies represents a clear advantage in optimization of submerged bioprocesses with filamentous organisms.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Similar content being viewed by others

References

  1. Adams HL, Thomas CR (1988) Biotechnol Bioeng 32:707

    CAS  Google Scholar 

  2. Packer HL, Thomas CR (1990) Biotechnol Bioeng 35:870

    CAS  Google Scholar 

  3. Thomas CR (1992) TIBTECH 10:343

    CAS  Google Scholar 

  4. Cox PW, Thomas CR (1992) Biotechnol Bioeng 32:945

    Google Scholar 

  5. Reichl U, King R, Gilles ED (1992) Biotechnol Bioeng 39:164

    CAS  Google Scholar 

  6. Paul GC, Thomas CR (1998) Adv Bioechem Eng/Biotechnol 60:1

    CAS  Google Scholar 

  7. Agger T, Spohr AB, Carlsen M, Nielsen J (1998) Biotechnol Bioeng 57:321

    CAS  Google Scholar 

  8. Krabben P, Nielsen J (1998) Adv Biochem Eng/Biotechnol 60:125

    Google Scholar 

  9. McIntyre M, Berry DR, McNeil B (1999) Enzyme Microbial Technol 25:447

    CAS  Google Scholar 

  10. Cui YQ, van der Lans RGJM, Luyben KCAM (1998) Biotechnol Bioeng 57:409

    CAS  Google Scholar 

  11. Paul GC, Kent CA, Thomas CR (1994) Trans I Chem E 72:95

    Google Scholar 

  12. Müller C, Spohr AB, Nielsen J (2000) Biotechnol Bioeng 67:390

    Google Scholar 

  13. Cox PW, Thomas CR (1999) Mycol Res 103:757

    Google Scholar 

  14. Bachewich C, Heath IB (1998) J Cell Sci 111:2005

    CAS  Google Scholar 

  15. Wolkow TD, Harris SD, Hamer JE (1996) J Cell Sci 109:2179

    CAS  Google Scholar 

  16. Christiansen T, Spohr AB, Nielsen J (1999) Biotechnol Bioeng 63:147

    CAS  Google Scholar 

  17. Spohr A, Dam-Mikkelsen C, Carlsen M, Nielsen J, Villadsen J (1998) Biotechnol Bioeng 58:541

    CAS  Google Scholar 

  18. Nielsen J, Villadsen J (1994) Bioreaction engineering principles. Plenum Press, New York

    Google Scholar 

  19. Heath IB (1995) Can J Bot 73:S131

    Google Scholar 

  20. Gow NAR (1995) Tip growth and polarity. In: Gow NAR, Gadd GM (eds) The growing fungus. Chapman and Hall, London, p 277

    Google Scholar 

  21. Wessels JGH (1993) Exp Phytol 123:397

    CAS  Google Scholar 

  22. Sietsma JH, Wessels JGH (1991) Cell wall assembly in fungal morphogenesis. In: Latgé JP, Boucias D (eds) Fungal cell wall and immune response. Springer, Berlin Heidelberg New York, p 81

    Google Scholar 

  23. McKerracher LJ, Heath IB (1987) Exp Mycol 11:79

    Google Scholar 

  24. Jackson SL, Heath IB (1992) Protoplasma 170:46

    Google Scholar 

  25. Heath IB (1990) Int Rev Cytol 123:95

    CAS  Google Scholar 

  26. Riquelme M, Reynaga-Pena CG, Gierz G, Bartnicki-Garcia S (1999) Fungal Genet Biol 24:101

    Google Scholar 

  27. Roberson RW, Fuller MS (1988) Protoplasma 146:143

    Google Scholar 

  28. Howard RJ, Aist JR (1979) J Ultrastr Res 66:224

    CAS  Google Scholar 

  29. Grove SN, Bracker CE (1970) J Bacteriol 104:989

    CAS  Google Scholar 

  30. Parton RM, Fischer S, Malhó R, Papasouliotis O, Jelitto TC, Leonard T, Read ND (1997) J Cell Sci 110:1187

    CAS  Google Scholar 

  31. Jackson SL, Heath IB (1993) Protoplasma 173:23

    CAS  Google Scholar 

  32. Robson GD, Wiebe MG, Trinci APJ (1991) J GenMicrobiol 137:963

    CAS  Google Scholar 

  33. Borgia PT, Dodge CL (1992) J Bacteriol 174:377

    CAS  Google Scholar 

  34. Gooday GW (1971) J Gen Microbiol 67:125

    CAS  Google Scholar 

  35. Bartnicki-Garcia S, Lippman E (1969) Science 165:302

    CAS  Google Scholar 

  36. Trinci APJ (1978) The duplication cycle and vegetative development in moulds. In: Smith JE, Berry DR (eds) The filamentous fungi, vol 3. Arnold, London, p 132

    Google Scholar 

  37. Kaminskyj SGW, Hamer JE (1998) Genetics 148:669

    CAS  Google Scholar 

  38. Wessels JGH (1990) Role of cell wall architecture in fungal tip growth generation. In: Heath IB (ed) Tip growth in plant and fungal cells. Academic Press, New York, p 1

    Google Scholar 

  39. Harris SD, Hofmann AF, Tedford HW, Maurice PL (1999) Genetics 151:1015

    CAS  Google Scholar 

  40. Mata J, Nurse P (1998) Trends Cell Biol 8:163

    CAS  Google Scholar 

  41. Schmidt A, Hall MN (1998) Ann Rev Cell Dev Biol 14:305

    CAS  Google Scholar 

  42. Torralba S, Raudaskoski M, Pedregosa AM, Laborda F (1998) Microbiology 144:45

    CAS  Google Scholar 

  43. Reissig JL, Kinney SG (1983) J Bacteriol 154:1397

    CAS  Google Scholar 

  44. Rasmussen H, Goodman DBP (1977) Physiol Rev 57:421

    CAS  Google Scholar 

  45. Katz D, Goldstein D, Rosenberger RF (1972) J Bacteriol 109:1097

    CAS  Google Scholar 

  46. Suelmann R, Sievers N, Fischer R (1997) Mol Microbiol 25:757

    CAS  Google Scholar 

  47. Xiang X, Beckwith SM, Morris R (1994) Proc Natl Acad Sci USA 91:2100

    CAS  Google Scholar 

  48. Xiang X, Morris R (1999) Curr Opinion Microbiol 2:636

    CAS  Google Scholar 

  49. Robb MJ, Wilson MA, Vierula PJ (1995) Mol Gen Genet 24:583

    Google Scholar 

  50. Nielsen J (1995) Physiological engineering aspects of Penicillium chrysogenum. DSc Thesis, Technical University of Denmark

    Google Scholar 

  51. Nielsen J, Carlsen M (1996) Fungal pellets. In: Willaert RG, Baron GV, De Backer L (eds) Immobilised living cell systems: modelling and experimental methods. Wiley, New York, p273

    Google Scholar 

  52. Spohr A, Agger T, Carlsen M, Nielsen J (1998) Quantitative morphology of filamentous organisms. In: Wilkinson MHF, Schut F (eds) Digital image analysis of microbes: imaging, morphometry, fluorometry and motility techniques and applications. Wiley, New York, p 373

    Google Scholar 

  53. Carlsen M, Spohr AB, Nielsen J, Villadsen J (1996) Biotechnol Bioeng 49:266

    CAS  Google Scholar 

  54. Paul GC, Kent CA, Thomas CR (1994) Trans I Chem E 72C:95

    Google Scholar 

  55. Tucker KG, Thomas CR (1993) Trans I Chem E 171:111

    Google Scholar 

  56. Braun S, Vecht-Lifshitz SE (1991) TIBTECH 9:63

    Google Scholar 

  57. Shamlou PA, Makagiansar HY, Ison AP, Lilly MD, Thomas CR (1994) Chem Eng Sci 49:2621

    Google Scholar 

  58. Makagiansar HY, Shamlou PA, Thomas CR, Lilly MD (1993) Bioprocess Eng 9:83

    CAS  Google Scholar 

  59. Smith JJ, Lilly MD, Fox RI (1990) Biotechnol Bioeng 35:1011

    CAS  Google Scholar 

  60. Paul GC, Kent CA, Thomas CR (1992) Trans I Chem E 70:13

    CAS  Google Scholar 

  61. Vanhoutte B, Pons MN, Thomas CR, Louvel L, Vivier H (1995) Biotechnol Bioeng 48:1

    CAS  Google Scholar 

  62. Sietsma JH, Wösten HAB, Wessels JGH (1995) Can J Bot 73: S388

    CAS  Google Scholar 

  63. Peberdy JF (1994) TIBTECH 12:50

    CAS  Google Scholar 

  64. Wösten HAB, Moukha SM, Sietsma JH, Wessels JGH (1991) J Gen Microbiol 137:2017

    Google Scholar 

  65. Wongwicharn A, McNeil B, Harvey LM (1999) Biotechnol Bioeng 65:416

    CAS  Google Scholar 

  66. Johansen CL, Coolen L, Hunik JH (1998) Biotechnol Prog 14:233

    CAS  Google Scholar 

  67. Bocking SP, Wiebe MG, Robson GD, Hansen K, Christiansen LH, Trinci APJ (1999) Biotechnol Bioeng 65:638

    CAS  Google Scholar 

  68. Spohr A, Carlsen M, Nielsen J, Villadsen J (1997) Biotechnol Letts 19:257

    CAS  Google Scholar 

  69. Fiddy C, Trinci APJ (1971) J Gen Microbiol 97:169

    Google Scholar 

  70. Amanullah A, Blair R, Nienow AW, Thomas CR(1999) Biotechnol Bioeng 62:434

    CAS  Google Scholar 

  71. Mitard A, Riba JP (1988) Biotechnol Bioeng 32:835

    CAS  Google Scholar 

  72. Metz B, Kossen NWF (1977) Biotechnol Bioeng 19:781

    CAS  Google Scholar 

  73. Riley GL, Tucker KG, Paul GC, Thomas CR (2000) Biotechnol Bioeng 68:160

    CAS  Google Scholar 

  74. Gordon CL, Khalaj V, Ram AFJ, Archer DB, Brookman JL, Trinci APJ, Jeenes DJ, Doonan JH, Wells B, Punt PJ, van den Hondel CAMJJ, Robson G (2000) Microbiology 146:415

    CAS  Google Scholar 

  75. Henriksen AS, Even S, Müller C, Punt PJ, van den Hondel CAMJJ, Nielsen J (1999) Microbiology 145:729

    CAS  Google Scholar 

  76. Harris SD, Hamer L, Sharpless KE, Hamer JE (1997) EMBOJ 16:3474

    CAS  Google Scholar 

  77. Momany M, Westfall PJ, Abramowsky G (1999) Genetics 151:557

    CAS  Google Scholar 

  78. Stark MJ (1996) Yeast 12:1647

    CAS  Google Scholar 

  79. Dickman MB, Yarden O (1999) Fungal Genet Biol 26:99

    CAS  Google Scholar 

  80. Madhani HD, Fink GR (1998) Trends Cell Biol 8:348

    CAS  Google Scholar 

  81. Bruno KS, Aramayo R, Minke PF, Metzenberg RL, Plamann M (1996) EMBO J 15:5772

    CAS  Google Scholar 

  82. Lee IH, Walline RG, Plamann M (1998) Mol Microbiol 29:209

    CAS  Google Scholar 

  83. Rudall KM (1969) J Polymer Science 28X:83

    Google Scholar 

  84. Kang MS, Elango N, Mattia E, Au-Young J, Robbins PW, Cabib E (1984) J Biol Chem 259:14, 966

    Google Scholar 

  85. Burnett JH (1979) Aspects of the structure and growth of hyphal walls. In: Burnett JH, Trinci APJ (eds) Fungal walls and hyphal growth. Cambridge University Press, Cambridge, p 1

    Google Scholar 

  86. Fujiwara M, Horiuchi H, Ohta A, Takagi M (1997) Biochem Biophys Res Commun, 236:75

    Google Scholar 

  87. Bowen AR, Chen-Wu JL, Momany M, Young R, Szaniszlo PJ, Robbins PW (1992) Proc Natl Acad Sci USA 89:519

    CAS  Google Scholar 

  88. Horiuchi H, Takagi M (1999) Chitin synthase genes of Aspergillus species. In: Brakhage AA, Jahn B, Schmidt A (eds) Aspergillus fumigatus: Contrib Microbiol. Basel Karger, Basel, p 193

    Google Scholar 

  89. Borgia PT, Iartchouk N, Riggle PJ, Winter KR, Koltin Y, Bulawa CE (1996) Fungal Genet Biol 20:193

    CAS  Google Scholar 

  90. Horiuchi H, Fujiwara M, Yamashita S, Otha A, Takagi M (1999) J Bacteriol 181:3721

    CAS  Google Scholar 

  91. De Marini DJ, Adams AEM, Fares H, De Virgilio C, Valle G, Vhuang JS, Pringle JR (1997) J Cell Biol 139:75

    Google Scholar 

  92. Choi W-J, Santos B, Duran A, Cabib E (1994) Mol Cell Biol 14:7685

    CAS  Google Scholar 

  93. Mellado E, Aufauvre-Brown A, Specht CA, Robbins PW, Holden DW (1996) Mol Gen Genet 246:353

    Google Scholar 

  94. Iten W, Matile P (1970) J Gen Microbiol 62:203

    Google Scholar 

  95. Horsch M, Rast DM (1993) Allosteric activation of chitin synthase by N-acetylglu-cosamine: a mechanistic study. In: Muzzarilli RAA (ed) Chitin enzymology. European Chitin Society, Ancona, Italy, p 47

    Google Scholar 

  96. Specht CA, Liu Y, Robbins PW, Bulawa CE, Iartchouk N, Winter KA, Riggle PJ, Rhodes JC, Dodge CL, Culp DW, Borgia PT (1996) Fungal Genet Biol 20:153

    CAS  Google Scholar 

  97. Culp DW, Dodge CL, Yihong M, Li L, Demet S-O, Borgia PT (2000) FEMS Microbiol Lett 182:349

    CAS  Google Scholar 

  98. Motoyama T, Sudoh M, Horiuchi H, Ohta A, Takagi M (1994) Biosci Biotech Biochem 58:1685

    CAS  Google Scholar 

  99. Motoyama T, Fujiwara M, Kojima N, Horiuchi H, Ohta A, Takagi M (1997) Mol Gen Genet 253:520

    CAS  Google Scholar 

  100. Yanai K, Kojima N, Takaya N, Horiuchi H, Ohta A, Takagi M (1994) Biosci Biotech Biochem 58:1828

    CAS  Google Scholar 

  101. Northrop FD, Ljubojevic S, Davies JM (1997) Microbiology 143:3757

    CAS  Google Scholar 

  102. Gow NAR (1997) Curr Top Med Mycol 8:43

    CAS  Google Scholar 

  103. Orlowski M (1991) Microbiol Rev 55:234

    CAS  Google Scholar 

  104. Odds FC (1985) Crit Rev Microbiol 12:45

    CAS  Google Scholar 

  105. Bartnicki-Garcia S (1968) J Bacteriol 96:1586

    CAS  Google Scholar 

  106. Madden K, Snyder M (1998) Ann Rev Microbiol 52:687

    CAS  Google Scholar 

  107. Brown CM, Hough JS (1965) Nature 206:676

    CAS  Google Scholar 

  108. Kron SJ, Gow NAR (1995) Curr Opin Cell Biol 7:845

    CAS  Google Scholar 

  109. Hoover CI, Jantaopour MJ, Newport G, Agabian N, Fisher SJ (1998) FEMS Microbiol Lett 167:163

    CAS  Google Scholar 

  110. Manavanthu E, Duncan C, Porte Q, Gunasekaran M (1996) Mycopathologia 135:79

    Google Scholar 

  111. Rickenberg HV(1974) Annu Rev Microbiol 28:353

    Google Scholar 

  112. Robinson GA, Butcher RW, Sutherland EW (1971) Cyclic AMP. Academic Press, New York

    Google Scholar 

  113. Dürrenberger F, Kronstad J (1999) Mol Gen Genet 261:281

    Google Scholar 

  114. Dürrenberger F, Wong K, Kronstad J (1998) Proc Natl Acad Sci USA 95:5684

    Google Scholar 

  115. Sonneborn A, Bockmuhl DP, Gerads M, Kurpanek K, Sanglard D, Ernst JF (2000) Mol Microbiol 35:386

    CAS  Google Scholar 

  116. Rossi S, Moreno S (1994) Eur J Biochem 222:501

    CAS  Google Scholar 

  117. Alonso-Monge R, Navarro-García F, Molero G, Diez-Orejas R, Gustin M, Pla J, Sánchez M, Nombela C (1999) J Bacteriol 181:3058

    CAS  Google Scholar 

  118. Navarro-García F, Alonso-Monge R, Rico H, Pla J, Sentandreu R, Nombela C (1998) Microbiology 144:411

    Google Scholar 

  119. McLain N, Dolan JW (1997) Microbiology 143:3521

    CAS  Google Scholar 

  120. Larsen AD, Sypherd PS (1974) J Bacteriol 117:432

    CAS  Google Scholar 

  121. Paveto C, Epstein A, Passeron S (1975) Mol Cell Biochem 65:55

    Google Scholar 

  122. Peters J, Sypherd PS (1979) J Bacteriol 137:1137

    Google Scholar 

  123. Inderlied CB, Cihlar RL, Sypherd PS (1980) J Bacteriol 141:699

    CAS  Google Scholar 

  124. Herrero AB, López MC, García S, Schmidt A, Spaltmann F, Ruiz-Herrera J, Dominguez A (1999) Infect Immun 67:4870

    CAS  Google Scholar 

  125. Guevara-Olvera L, Xonocostle-Cázares B, Ruiz-Herrera J (1997) Microbiology 143: 2237

    CAS  Google Scholar 

  126. Bartnicki-Garcia S, Nickerson WJ (1962) J Bacteriol 84:841

    CAS  Google Scholar 

  127. Sypherd PS, Borgia PT, Paznokas JL (1978) Adv Microbial Phys 18:67

    CAS  Google Scholar 

  128. Gordon PA, Stewart PR, Clark-Walker GD (1971) J Bacteriol 107:114

    CAS  Google Scholar 

  129. Safe S, Caldwell J (1975) Can J Microbiol 21:79

    CAS  Google Scholar 

  130. Chen-Wu JL, Zwicker J, Bowen AR, Robbins PW (1992) Mol Microbiol 6:497

    CAS  Google Scholar 

  131. Munro CA, Schofield DA, Gooday GW, Gow NAR (1998) Microbiology 144:391

    CAS  Google Scholar 

  132. Domek DB, Borgia PT (1981) J Bacteriol 146:945

    CAS  Google Scholar 

  133. Lopez-Matas MA, Eslava AP, Diaz-Minguez JM (2000) Curr Microbiol 40:169

    CAS  Google Scholar 

  134. Xonocostle-Cazares B, Specht CA, Robbins PW, Liu Y, León C, Ruiz-Herrera J (1997) Fungal Genet Biol 22:199

    Google Scholar 

  135. Sabanero M, Zazueta R (1989) FEMS Microbiol Lett 60:227

    CAS  Google Scholar 

  136. Hasek J, Bartnicki-Garcia S (1994) Arch Microbiol 161:363

    CAS  Google Scholar 

  137. Yokoyama K, Kaji H, Miyaji M (1990) J Gen Microbiol 136:1067

    CAS  Google Scholar 

  138. Akashi T, Kanve T, Tanaka K (1994) Microbiology 140:271

    Google Scholar 

  139. Steinberg G, Schliwa M, Lehmler C, Bölker M, Kahmann R, McIntosh JR (1998) J Cell Sci 111:2235

    CAS  Google Scholar 

  140. Torres-Guzman JC, Dominguez A (1997) Mol Cell Biol 17:6283

    CAS  Google Scholar 

  141. Braun BR, Johnson AD (1997) Science 277:105

    CAS  Google Scholar 

  142. Gale CA, Bendel CM, McClellan M, Hauser M, Becker JM, Berman J, Hostletter MK (1998) Science 27:1355

    Google Scholar 

  143. Saporito-Irwin SM, Birse CE, Sypherd PS, Fonzi WA (1995) Mol Cell Biol 15:601

    CAS  Google Scholar 

  144. Stoldt VR, Sonneborn A, Leuker CE, Ernst JF (1997) EMBOJ 16:1982

    CAS  Google Scholar 

  145. Yaar L, Mevarech M, Koltin Y (1997) Microbiology 143:3033

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Center for Process Biotechnology, Department of Biotechnology, Building 223, Technical University of Denmark, 2800, Lyngby, Denmark

    Mhairi McIntyre, Christian Müller, Jens Dynesen & Jens Nielsen

Authors
  1. Mhairi McIntyre
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Christian Müller
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jens Dynesen
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jens Nielsen
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Center for Process Biotechnology Department of Biotechnology Building 223, Technical University of Denmark, 2800, Lyngby, Denmark

    Jens Nielsen

Rights and permissions

Reprints and permissions

Copyright information

© 2001 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

McIntyre, M., Müller, C., Dynesen, J., Nielsen, J. (2001). Metabolic Engineering of the Morphology of Aspergillus . In: Nielsen, J., et al. Metabolic Engineering. Advances in Biochemical Engineering/Biotechnology, vol 73. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3-540-45300-8_6

Download citation

  • DOI: https://doi.org/10.1007/3-540-45300-8_6

  • Received:

  • Published:

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-41848-1

  • Online ISBN: 978-3-540-45300-0

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation