Abstract
In spite of the enormous possibilities of macromolecules as key elements in developing advancedmaterials with increased functionality and complexity, the success in this development is often limitedby the randomness associated with polymer synthesis and the exponential increase in technical difficultiescaused by the attempt to reach a sufficiently high degree of complexity in the molecular design.This paper describes a new approach in the design of complex and highly functional macromolecules,the genetic engineering of protein-based macromolecules. The exploitation of the efficient machineryof protein synthesis in living cells opens a path to obtain extremely well-defined and complexmacromolecules. Different molecular designs are presented, with increasing degree of complexity,showing how the controlled increase in their complexity yields (multi)functional materials with moreselect and sophisticated properties. The simplest designs show interesting properties already, butthe adequate introduction of given chemical functions along the polymer chain presents an opportunityto expand the range of properties to enhanced smart behavior and self-assembly. Finally, examplesare given where those molecular designs further incorporate selected bioactivities in order to developmaterials for the most cutting-edge applications in the field of biomedicine and nano(bio)technology.
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Pintauer T, Matyjaszewski K (2005) Coordin Chem Rev 249:1155
Pyun J, Tang CB, Kowalewski T, Frechet JMJ, Hawker CJ (2005) Macromolecules 38:2674
Nyce GW, Glauser T, Connor EF, Mock A, Waymouth RM, Hedrick JL (2003) J Am Chem Soc 125:3046
Coates GW, Moore DR (2004) Angew Chem Int Edit 43:6618
Deming TJ (2002) Adv Drug Deliv Rev 54:1145
United Nations Framework Convention on Climate Change ( http://unfccc.int/2860.php )
Massimini K (ed) (2001) Genetic disorders sourcebook. Omnigraphics, Detroit, MI
Cappello J (1992) MRS Bull 17:48
McGrath K, Kaplan D (eds) (1997) Protein-based materials. Birkhäuser, Boston
Krejchi MT, Atkins EDT, Waddon AJ, Fournier MJ, Mason TL, Tirrell DA (1994) Science 265:1427
Capello J, Ferrari F (1994) In: Mobley DP (ed) Plastics from microbes. Hanser=Gardner, Cincinnati, OH, p 35
McMillan RA, Lee TAT, Conticello VP (1999) Macromolecules 32:3643
Meyer DE, Chilkoti A (2002) Biomacromolecules 3:357
McPherson DT, Morrow C, Minehan DS, Wu JG, Hunter E, Urry DW (1992) Biotechnol Prog 8:347
Prince JT, McGrath KP, Digirolamo CM, Kaplan DL (1995) Biochemistry 34:10879
Guda C, Zhang X, McPherson DT, Xu J, Cherry JH, Urry DW, Daniell H (1995) Biotechnol Lett 17:745
Whaley SR, English DS, Hu EL, Barbara PF, Belcher AM (2000) Nature 405:665
Lee J, Macosko CW, Urry DW (2001) Biomacromolecules 2:170
Girotti A, Reguera J, Arias FJ, Alonso M, Testera AM, Rodríguez-Cabello JC (2004) Macromolecules 37:3396
Meyer DE, Chilkoti A (2004) Biomacromolecules 5:846
Park C, Yoon J, Thomas EL (2003) Polymer 44:6725
Elices M (2000) Structural biological materials: design and structure-property pelationships. Elsevier, London
Hinman MB, Jones JA, Lewis RV (2000) Tibtech 18:374
Vollrath F, Knight DP (2001) Nature 410:541
Shao Z, Vollrath F (2002) Nature 418:741
Tatham AS, Shewry PR (2000) Tibs 25:567
Gosline J, Lillie M, Carrington E, Guerette P, Ortlepp C, Savage K (2002) Philos Trans R Soc Lond B 357:121
Lombardi EC, Kaplan DL (1993) Mater Res Soc Symp Proc 292:3
Urry DW, Hugel T, Seitz M, Gaub HE, Sheiba L, Dea J, Xu J, Parker T (2002) Phil Trans R Soc Lond B 357:169
Urry DW (2005) What sustains life? Consilient mechanisms for protein-based machines and materials. Springer, Berlin Heidelberg New York
Urry DW (2005) Deciphering engineering principles for the design of protein-based nanomachines. In: Renugopalakrishnan V, Lewis R (eds) Protein-based nanotechnology. Kluwer, Dordrecht (in press)
Kwon I, Kirshenbaum K, Tirrell DA (2003) J Am Chem Soc 125:7512
Yu BY (2002) Adv Drug Deliv Rev 54:1113
Bilgiçer B, Fichera A, Kumar K (2001) J Am Chem Soc 123:4393
Tang Y, Ghirlanda G, Vaidehi N, Kua J, Mainz DT, Goddard WA, DeGrado WF, Tirrell DA (2001) Biochemistry 40:2790
Potekhin SA, Medvedkin VN, Kashparov IA, Venyaminov S (1994) Protein Eng 7:1097
Goeden-Wood NL, Keasling JD, Muller SJ (2003) Macromolecules 36:2932
Panitch A, Matsuki K, Cantor EJ, Cooper SJ, Atkins EDT, Fournier MJ, Mason TL, Tirrell DA (1997) Macromolecules 30:42
Zhang G, Fournier MJ, Mason TL, Tirrell DA (1992) Macromolecules 25:3601
Chilkoti A, Dreher MR, Meyer DE (2002) Adv Drug Deliv Rev 54:1093
Haider M, Megeed Z, Ghandehari H (2004) J Control Release 95:1
Gosline JM, DeMont ME, Denny MW (1986) Endeavour 10:31
Gosline JM, Guerette PA, Ortlepp CS, Savage KN (1999) J Exp Biol 202:3295
Vollrath F (1992) Sci Am 266:70
Vollrath F (2000) J Biotechnol 74:67
Gatesy J, Hayashi C, Motriuk D, Woods J, Lewis R (2001) Science 291:2603
Scheibel T (2004) Microb Cell Fact 3:14
Colgin MA, Lewis RV (1998) Protein Sci 7:667
Hayashi CY, Blackledge TA, Lewis RV (2004) Mol Biol Evol 21:1950
Guerette PA, Ginzinger DG, Weber BH, Gosline JM (1996) Science 272:112
Hinman MB, Lewis RV (1992) J Biol Chem 267:19320
Craig CL, Riekel C (2002) Comp Biochem Physiol B Biochem Mol Biol 133:493
Sponner A, Unger E, Grosse F, Weisshart K (2004) Biomacromolecules 5:840
Xu M, Lewis RV (1990) Proc Natl Acad Sci USA 87:7120
Hayashi CY, Lewis RV (1998) J Mol Biol 275:773
Beckwitt R, Arcidiacono S (1994) J Biol Chem 269:6661
Simmons AH, Ray E, Jelinski LW (1994) Macromolecules 27:5235
Parkhe AD, Seeley SK, Gardner K, Thompson L, Lewis RV (1997) J Mol Recog 10:1
Bram A, Branden CI, Craig C, Snigireva I, Riekel C (1997) J Appl Cryst 30:390
Simmons AH, Michal CA, Jelinski LW (1996) Science 271:84
Kummerlen J, Vanbeek J, Vollrath F, Meier B (1996) Macromolecules 29:2920
Lewis RV, Hinman M, Kothakota S, Fournier MJ (1996) Protein Expr Purif 7:400
Hutchinson E, Thornton J (1994) Protein Sci 3:2207
Urry DW, Luan CH, Peng SQ (1995) Ciba Found Symp 192:4
Van Dijk AA, Van Wijk LL, Van Vliet A, Haris P, Van Swieten E, Tesser GI, Robillard GT (1997) Protein Sci 6:637
Van Beek JD, Hess S, Vollrath F, Meier BH (2002) Proc Natl Acad Sci USA 99:10266
Dong Z, Lewis RV, Middaugh CR (1991) Arch Biochem Biophys 284:53
Fahnestock SR, Yao Z, Bedzyk LA (2000) J Biotechnol 74:105
Huemmerich D, Helsen CW, Quedzuweit S, Oschmann J, Rudolph R, Scheibel T (2004) Biochemistry 43:13604
Cappello J, Ferrari F (1994) In: Mobley DP (ed) Plastics from microbes. Hanser, New York, p 35
Nagarsekar A, Crissman J, Crissman M, Ferrari F, Cappello J, Ghandehari H (2002) J Biomed Mater Res 62:195
Ferrari F, Richardson C, Chambers J, Causey SC, Pollock TJ, Cappello J, Crissman JW (1987) US Patent 5,243,038
Urry DW, Pattanaik A, Xu J, Woods TC, McPherson DT, Parker TM (1998) J Biomater Sci Polym Edn 9:1015
Lee J, Macosko CW, Urry DW (2001) J Biomater Sci Polym Edn 12:229
Nicol A, Gowda DC, Parker TM, Urry DW (1993) J Biomed Mater Res 27:801
Urry DW (2003) Elastic protein-based biomaterials: elements of basic science, controlled release and biocompatibility. In: Wise DL, Hasirci V, Yaszemski MJ, Altobelli DE, Lewandrowski KU, Trantolo DJ (eds) Biomaterials handbook–advanced applications of basic sciences and bioengineering. Marcel Dekker, New York
Meyer DE, Kong GA, Dewhirst MW, Zalutsky MR, Chilkoti A (2001) Cancer Res 61:1548
Trabbic-Carlson K, Setton LA, Chilkoti A (2003) Biomacromolecules 4:572
Knight MK, Setton LA, Chilkoti A (2003) Summer Bioengineering Conference, Key Biscayne, FL, 25–29 June 2003
Dreher MR, Raucher D, Balu N, Colvin OM, Ludeman SM, Chilkoti A (2003) J Control Release 91:31
Hyun J, Lee WK, Nath N, Chilkoti A, Zauscher S (2004) J Am Chem Soc 126:7330
Trabbic-Carlson K, Liu L, Kim B, Chilkoti A (2004) Protein Sci 13:3274
Deguchi Y, Fournier MJ, Mason TL, Tirrell DA (1994) JMS-Pure Appl Chem A 31:1691
Panitch A, Yamaoka T, Fournier MJ, Mason TL, Tirrell DA (1999) Macromolecules 32:1701
Di Zio K, Tirrell DA (2003) Macromolecules 36:1553
McGrath KP, Fournier MJ, Mason TL, Tirrell DA (1992) J Am Chem Soc 114:727
Lumb KJ, Kim PS (1995) Biochemist 34:8642
Girotti A, Reguera J, Rodríguez-Cabello JC, Arias FJ, Alonso M, Testera AM (2004) J Mater Sci Mater M 15:479
Dinerman AA, Cappello J, Ghandehari H, Hoag SW (2002) Biomaterials 23:4203
Nagarsekar A, Crissman J, Crissman M, Ferrari F, Cappello J, Ghandehari H (2003) Biomacromolecules 4:602
Cappello J, Crissman JW, Crissman M, Ferrari FA, Textor G, Wallis O, Whitledge JR, Zhou X, Burman D, Aukerman L, Stedronsky ER (1998) J Control Release 53:105
Zhou Y, Wu S, Conticello VP (2001) Biomacromolecules 2:111
Wright ER, Conticello VP (2002) Adv Drug Deliv Rev 54:1057
Nagapudi K, Brinkman WT, Leisen J, Thomas BS, Wright ER, Haller C, Wu X, Apkarian RP, Conticello VP, Chaikof EL (2005) Macromolecules 38:345
Ohgo K, Kurano TL, Kumashiro KK, Asakura T (2004) Biomacromolecules 5:744
Asakura T, Nitta K, Yang M, Yao J, Nakazawa Y, Kaplan DL (2003) Biomacromolecules 4:815
O'Brien JP, Fahnestock SR, Termonia Y, Gardner KH (1998) Adv Mater 10:1185
Goldberg I, Salerno AJ, Patterson T, Williams JI (1989) Gene 80:305
Shimazu M, Mulchandani A, Chen W (2003) Inc Biotechnol Bioeng 81:74
Ayad S, Humphries M, Boot-Handford R, Kadler K, Shuttleworth A (1994) The extracellular matrix facts book. Facts Book Series. Academic, San Diego
Urry DW, Luan CH, Harris CM, Parker T (1997) Protein-based materials with a profound range of properties and applications: the elastin ∆T t hydrophobic paradigm. In: McGrath K, Kaplan D (eds) Proteins and modified proteins as polymeric materials. Birkhäuser, Boston, p 133–177
Urry DW (1998) Biopolymers 47:167
Gowda DC, Parker TM, Harris RD, Urry DW (1994) Synthesis, characterization and medical applications of bioelastic materials. In: Basava C, Anantharamaiah GM (eds) Peptides: design, synthesis and biological activity. Birkhäuser, Boston, p 81
Martino M, Perri T, Tamburro AM (2002) Macromol Biosci 2:319
Urry DW (1993) Angew Chem Int Edit Engl 32:819
San Biagio PL, Madonia F, Trapane TL, Urry DW (1988) Chem Phys Lett 145:571
Urry DW (1997) J Phys Chem B 101:11007
Rodríguez-Cabello JC, Alonso M, Pérez T, Herguedas MM (2000) Biopolymers 54:282
Tanford C (1973) The hydrophobic effect: formation of micelles and biological membranes. Wiley, New York
Pauling L, Marsh E (1952) Proc Natl Acad Sci USA 38:112
Urry DW, Trapane TL, Prasad KU (1985) Biopolymers 24:2345
Manno M, Emanuele A, Martorana V, San Biagio PL, Bulone D, Palma-Vittorelli MB, McPherson DT, Xu J, Parker TM, Urry DW (2001) Biopolymers 59:51
Alonso M, Arranz D, Reboto V, Rodríguez-Cabello JC (2001) Macromol Chem Phys 202:3027
Reguera J, Lagaron JM, Alonso M, Reboto V, Calvo B, Rodríguez-Cabello JC (2003) Macromolecules 36:8470
Rodríguez-Cabello JC, Reguera J, Alonso M, Parker TM, McPherson DT, Urry DW (2004) Chem Phys Lett 388:127
Reading M (1993) Trends Polym Sci 1:248
Wunderlich B, Androsch R, Pyda M, Kwon YK (2000) Thermochim Acta 348:181
Gill PS, Sauerbrunn SR, Reading M (1993) J Therm Anal 40:931
Jorimann U, Widmann G, Riesen R (1999) J Therm Anal Calor 56:639
Menczel JD, Judovist L (1998) J Therm Anal 54:419
Rodríguez-Cabello JC, Alonso M, Guiscardo L, Reboto V, Girotti A (2002) Adv Mater 14:1151
Alonso M, Reboto V, Guiscardo L, San Martín A, Rodríguez-Cabello JC (2000) Macromolecules 33:9480
Alonso M, Reboto V, Guiscardo L, Mate V, Rodríguez-Cabello JC (2001) Macromolecules 34:8072
Yang G, Woodhouse KA, Yip CM (2002) J Am Chem Soc 124:10648
Lee TAT, Cooper A, Apkarian RP, Conticello VP (2000) Adv Mater 12:1105
Reguera J, Fahmi A, Moriarty P, Girotti A, Rodríguez-Cabello JC (2004) J Am Chem Soc 126:13212
Herrero-Vanrell R, Rincón A, Alonso M, Reboto V, Molina-Martinez I, Rodríguez-Cabello JC (2005) J Control Release 102:113
Nath N, Chilkoti A (2003) Anal Chem 75:709
Nath N, Chilkoti A (2001) J Am Chem Soc 123:8197
Nath N, Chilkoti A (2002) Adv Mater 14:1243
Urry DW (2004) Chem Phys Let 399:177
Ciardelli F, Fabbri D, Pieroni O, Fissi A (1989) J Am Chem Soc 111:3470
Stayton PS, Hoffman AS, Murthy N, Lackey C, Cheung C, Tan P, Klumb LA, Chilkoti A, Wilbur FS, Press OW (2000) J Control Release 65:203
Waite JH, Sun C, Lucas JM (2002) Philos Trans R Soc B 357:143
Chilkoti A, Dreher MR, Meyer DE, Raucher D (2002) Adv Drug Deliv Rev 54:613
Meyer DE, Shin BC, Kong GA, Dewhirst MW, Chilkoti A (2001) J Control Release 74:213
Kostal J, Mulchandani A, Chen W (2001) Macromolecules 34:2257
Kuwabara T, Nakamura A, Ueno A, Toda F (1994) J Phys Chem 98:6297
Chokchainarong S, Fennema OR, Connors KA (1992) Carbohyd Res 232:161
Reguera J, Alonso M, Testera AM, López IM, Martín S, Rodríguez-Cabello JC (2004) Carbohyd Polym 57:293
Urry DW, Parker TM, Reid MC, Gowda DC (1991) J Bioactive Comp Polym 6:263
Urry DW, Gowda DC, Harris CM, Harris RD (1994) Bioelastic materials and the ∆T t-Mechanism in drug delivery. In: Ottenbrite RM (ed) Polymeric Drugs and Drug Administration. ACS, Washintong DC, chap 2, p 15
Urry DW (1990) Polym Mater Sci Eng 63:329
Andersson L, Davies J, Duncan R, Ferruti P, Ford J, Kneller S, Mendichi R, Pasut G, Schiavon O, Summerford C, Tirk A, Veronese FM, Vincenzi V, Wu G (2005) Biomacromolecules 6:914
Jiuliano RL (2002) Annu Rev Pharmacol Toxicol 42:283
Sternlicht MD, Werb Z (2001) Annu Rev Cell Dev Biol 17:463
Urry DW, Nicol A, Gowda DC, Hoban LD, McKee A, Williams T, Olsen DB, Cox BA (1993) Medical applications of bioelastic materials. In: Gebelein CG (ed) Biotechnological polymers: medical, pharmaceutical and industrial applications. Technomic, Atlanta, p 82–103
Nicol A, Gowda DC, Parker M, Urry DW (1994) Cell adhesive properties of bioelastic materials containing cell attachment sequences. In: Gebelein C, Carraher C (eds) Biotechnology and bioactive polymers. Plenum, New York
Welsh ER, Tirrell DA (2000) Biomacromolecules 1:23
Nowatzki PJ, Tirrell DA (2004) Biomaterials 25:1261
Alix AJ (2001) J Soc Biol 195:181
Acknowledgments
This work was supported by the “Junta de Castilla y León” (VA002/02), by the MCYT (MAT2000-1764-C02, MAT2001-1853-C02-01, MAT2003-01205, and MAT2004-03484-C02-01), and by the European Commission (Marie Curie Research Training Network BioPolySurf MRTN-CT-2004-005516).
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Rodríguez-Cabello, J.C., Reguera, J., Girotti, A., Arias, F.J., Alonso, M. (2005). Genetic Engineering of Protein-Based Polymers: The Example of Elastinlike Polymers. In: Vancso, G.J. (eds) Ordered Polymeric Nanostructures at Surfaces. Advances in Polymer Science, vol 200. Springer, Berlin, Heidelberg. https://doi.org/10.1007/12_047
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