Abstract
Disomic wheat/barley addition lines were developed from hybrids produced with the German two-rowed winter barley cultivar ‘Igri’ and the Ukrainan six-rowed winter barley cultivar ‘Manas’. The 2H, 3H and 4H disomic addition lines of winter wheat ‘Martonvásári 9 kr1’/winter barley ‘Igri’ produced in Martonvásár were identified using GISH, FISH and SSR markers. A disomic addition of the 1HS isochromosome was also identified. The 2H, 3H and 4H addition lines have been multiplied in the nursery and characterized morphologically. Backcross progenies were produced and identified with molecular genetic and cytogenetic methods from the Japanese wheat ‘Asakaze komugi’ × Ukrainan six-rowed winter barley ‘Manas’ hybrids. A deletion and a translocated chromosome facilitated the physical localization of microsatellite markers on chromosome 5H. So far the 4H disomic addition line has been identified from the fertile BC2 plants in this combination, but the development of other disomic additions is in progress
Access provided by Autonomous University of Puebla. Download to read the full chapter text
Chapter PDF
Similar content being viewed by others
References
Gerlach WL,Bedbrook JR (1979)Cloning and characterization of ribosomal RNA genes from wheat and barley. Nucleic Acids Res 7:1869–1885
Islam AKMR,Shepherd KW,Sparrow DHB (1978) Production and characterization of wheat-barley addition lines. In: Ramunujam, S (ed) Proc. 5th Int. Wheat Genetics Symp, New Delhi, India, pp 356–371
Jensen J,Linde-Laursen I (1992)Statistical evaluation of length measurements on barley chromosomes with a proposal for new nomenclature for symbols and positions of cytological markers. Hereditas 117:51–59
Koba T,Takumi S,Shimada T (1997)Isolation, identification and characterization of disomic and translocated barley chromosome addition lines of common wheat. Euphytica 96:289–296
Kruse A (1973)Hordeum × Triticum hybrids. Hereditas 73:157–161
Leitch IJ,Heslop-Harrison JS (1992)Physical mapping of the 18S-5.8S-26S rRNA genes in barley by in situ hybridization. Genome 35:1013–1018
Linc G,Friebe BR,Kynast RG, Molnár-Láng M, Kõszegi B,Sutka J,Gill BS (1999)Molecular cytogenetic analysis of Aegilops cylindrica Host. Genome 42:497–503
Linc G,Molnár-Láng M (2003)Two methods for the development of wheat/barley disomic additions in winter wheat varieties, and identification using molecular cytogenetic techniques (C-banding, GISH). Növénytermelés 52:3–13
Molnár-Láng M,Linc G,Sutka J (1996)Transfer of the recessive crossability allele kr1from ‘Chinese Spring’ into the winter wheat variety Martonvàsàri 9. Euphytica 90:301–305
Molnár-Láng M,Linc G,Logojan A,Sutka J (2000)Production and meiotic pairing behaviour of new hybrids of winter wheat (Triticum aestivum) x winter barley (Hordeum vulgare). Genome 43:1045–1054
Molnár-Láng M,Novotny C,Linc G,Nagy ED (2005)Changes in the meiotic pairing behaviour of a winter wheat-winter barley hybrid maintained for a long term in tissue culture, and tracing the barley chromatin in the progenies using GISH and SSR markers. Plant Breed 124:247–252
Pedersen C,Langridge P (1997)Identification of the entire chromosome complement of bread wheat by two- colour FISH. Genome 40:589–593
Pedersen C,Rasmussen SK,Linde-Laursen I (1996)Genome and chromosome identification in cultivated barley and related species of the Triticeae (Poaceae) by in situ hybridization with the GAA-satellite sequence. Genome 39:93–104
Ramsay L,Macaulay M,degli Ivanissevich S,MacLean K,Cardle L,Fuller J,Edwards KJ,Tuvesson S,Morgante M,Massari A,Maestri E,Marmiroli N,Sjakste T,Ganal M,Powell W,Waugh R (2000)A simple sequence repeat-based linkage map of barley. Genetics 156:1997–2005
Rayburn AL,Gill BS (1986)Isolation of a D genome specific repeated DNA sequence from Aegilops squarrosa. Plant Mol Biol Rep 4:102–109
Reader SM,Abbo S,Purdie KA,King IP,Miller TE (1994)Direct labelling of plant chromosomes by rapid in situ hybridization. Trends Genet 10:265–266
Schubert I,Shi F,Fuchs J,Endo TR (1998)An efficient screening for terminal deletions and translocations of barley chromosomes added to common wheat. Plant J 14:489–495
Shepherd KW,Islam AKMR (1988) Fourth compendium of wheat-alien chromosome lines. In: Miller TE, Koebner RMD (eds) Proc 7th Int. Wheat Genet Symp, Cambridge, England, pp 1373–1398
Vràna J,Kubalàkovà M,Simkovà H,Cìhalìkovà J,Lysàk MA,Dolezel J (2000)Flow sorting of mitotic chromosomes in common wheat (Triticum aestivum L.). Genetics 156:2033–2041
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2007 Springer
About this paper
Cite this paper
Molnár-Láng, M., Szakács, É., Linc, G., Nagy, E.D. (2007). Production and Molecular Cytogenetic Identification of New Winter Wheat/Winter Barley Disomic Addition Lines. In: Buck, H.T., Nisi, J.E., Salomón, N. (eds) Wheat Production in Stressed Environments. Developments in Plant Breeding, vol 12. Springer, Dordrecht. https://doi.org/10.1007/1-4020-5497-1_86
Download citation
DOI: https://doi.org/10.1007/1-4020-5497-1_86
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-5496-9
Online ISBN: 978-1-4020-5497-6
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)