Abstract
Micronutrient deficiencies in human beings are common problems, especially in developing world. Among the micronutrient deficiencies, zinc (Zn) and iron (Fe) deficiencies are particularly important affecting severely health of humans. Major reason for the widespread occurrence of micronutrient deficiencies in human beings is the high and monotonous consumption of cereal-based foods with very low content of micronutrients. An increase in concentration of Zn and Fe in grain is, therefore, a high-priority research area. Exploitation of large genetic variation for Zn and Fe existing in cereals germplasm is an important approach to minimize the extent of Zn and Fe deficiencies in developing world. In the present study, the variation for seed content of micronutrients (Zn, Fe, Mn and Cu) in 54 accessions of einkorn wheat (Triticum monococcum) was tested. The accessions have been first grown under same field conditions in 2 locations in Turkey, and the seeds obtained from the field trials were analyzed for micronutrients. In addition, a mapping population with 168 recombinant inbred lines which were grown in 4 locations in Germany, Turkey and Italy has also been tested for the variation of micronutrients in seeds and analyzed for identification of QTLs associated with micronutrient content in seeds The results obtained showed existence of large genotypic variation in content of micronutrients. The contents of Zn and Fe among the 54 einkorn wheat accessions varied from 0.21 to 2.16 μg seed-1 for Zn with an average of 1.19 μg seed-1 and from 0.54 to 3.09 μg seed-1 for Fe with an average of 1.15 μg seed-1. There was a close positive relationship between seed contents of Fe and Zn. The genetic basis of this variation was elucidated by QTL analysis, using a mapping population comprising 168 recombinant inbred lines that was developed from a cross between 2 cultivated Einkorn genotypes (e.g., ID-362 bread-making quality poor and ID-331 bread-making quality good). From the parents ID-362 had always more Zn than the other parent in all four locations. The four locations presented different mean values, varying from 1.09 to 2.16 μg seed-1 for Zn content, from 0.83 to 1.97 μg seed-1 for Fe content, from 1.43 to 1.97 μg/seed-1 for Mn content and from 0.14 and to 0.24 μg seed-1 for Cu content. Pooling the results of the four trials, a major QTL, common to all four microelements and explaining from 10 to 30% of the variation (depending on the mineral assayed), was observed only on the chromosome 5, and not on the other chromosomes. The Einkorn germplasm tested had a significant variation for micronutrients, especially Zn and this variation could be exploited inbreeding programs. Chromosome 5 likely carries the genes affecting micronutrient accumulation in Einkorn seeds
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References
Acquistucci R, D’Egidio MG, Vallega V (1995) Amino acid composition of selected strains of diploid wheat, Triticum monococcum L. Cereal Chem 72:213–216
Bangizer M, Long J (2000) The potential for increasing the iron and zinc density of maize through plant breeding. Food Nutr Bull 21:397–400
Beebe S, Gonzalez AV, Rengifo J (2000) Research on trace minerals in the common bean. Food Nutr Bull 21:387–391
Borghi B, Castagna R, Corbellini M, Heun M, Salamini F (1996) Breadmaking quality of Einkorn wheat. Cereal Chem 73:208–214
Cakmak I, Ozkan H, Braun HJ, Welch RM, Romheld V (2000) Zinc and iron concentrations in seeds of wild, primitive and modern wheats. Food Nutr Bull 21(4):401–403
Cakmak I, Torun A, Millet E, Feldman M, Fahima T, Korol A, Nevo E, Braun HJ, Ozkan H (2004) Triticum dicoccoides: An important genetic resource for increasing zinc and iron concentration in modern cultivated wheat. Soil Sci Plant Nutr 50(7):1047–1054
Castagna R, Borghi B, Di Fonzo N, Heun M, Salamini F (1995) Yield and related of Einkorn (Triticum monococcum ssp. monococcum) in different environments. Eur J Agr 4:371–378
Graham RD, Senadhira D, Beede SE, Iglesias C, Ortiz-Monasterio I (1999) Breeding for micronutrient density in edible portions of staple food crops: conventional approaches. Field Crops Res 60:57–80
Gregorio GB, Senadhira H, Htut H, Graham RD (2000) Breeding for trace mineral density in rice. Food Nutr Bull 21:382–386
Hotz C, Brown KH (2004) Assessment of the risk of zinc deficiency in populations and options for its control. Food Nutr Bull 25:94
Ortiz-Monasterio I, Graham RD (2000) Breeding for trace minerals in wheat. Food Nutr Bull 21:392–396
Peterson CJ, Johnson VA, Mattern PJ (1986) Influence of cultivar and environment on mineral and protein concentrations of wheat flour, bran and grain. Cereal Chem 63:118–186
Rengel Z, Batten GD, Crowley DE (1999) Agronomic approaches for improving the micronutrient density in edible portions of staple field crops. Field Crops Res 60:27–40
Stam P, Van Ooijen JW (1995) Join Map Version 2.0. Software fort he calculation of genetic linkage maps. Wageningen, The Netherlands, CPRO-DLO
Taenzler B, Esposti RF, Vaccino P, Brandolini A, Effgen S, Heun M, Schafer-Pregl R, Borghi B, Salamini F (2002) Molecular linkage map of Einkorn wheat: mapping of storage-protein and soft-glume genes and bread-making quality QTLs. Genet Res 80:131–143
Utz HF, Melchinger AE (1996) PLABQTL: a program for composite interval mapping of QTL. Journal of Quantitative Trait Loci 2:1
Welch RM, Graham RD (1999) A new paradigm for world agriculture: meeting human needs. Productive, sustainable, nutritious. Field Crops Res 60:1–10
Vallega V (1979) Field performance of varieties of Triticum monococcum, T. durum, and Hordeum vulgare grown at two locations. Genet Agr 33:363–370
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Ozkan, H. et al. (2007). Natural Variation And Identification Of Microelements Content In Seeds Of Einkorn Wheat (Triticum Monococcum). In: Buck, H.T., Nisi, J.E., Salomón, N. (eds) Wheat Production in Stressed Environments. Developments in Plant Breeding, vol 12. Springer, Dordrecht. https://doi.org/10.1007/1-4020-5497-1_55
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DOI: https://doi.org/10.1007/1-4020-5497-1_55
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