Summary
A broad overview of the physiology and biochemistry of Rubisco is presented with a comparison of information obtained in vitro and in vivo. First a brief background to kinetic properties of Rubisco is given and Rubisco’s influence on photosynthetic metabolism is reviewed for C3, C4, CAM and C3-C4 species. The effect of environmental variables such as light and CO2 are considered for both short and longer term effects on the activity and abundance of Rubisco protein. Over the past few years experiments with transgenic plants with antisense RNA constructs to Rubisco, Rubisco activase and several of the PCR cycle enzymes have added new insights into Rubisco physiology. For example, transgenic tobacco with reduced amount of Rubisco has allowed the identification of environmental conditions where Rubisco exerts maximal control on photosynthesis. These plants have also been used to determine Rubisco kinetic constants in vivo. Transgenic plants with reduced amounts of Rubisco activase have been used to elucidate the role of activase in vivo.
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Abbreviations
- Γ:
-
CO2 compensation point
- Γ.:
-
CO2 compensation point in the absence of day respiration
- A:
-
rate of CO2 assimilation
- Ca, Cc, Ci:
-
partial pressure of CO2 in the air surrounding the leaf, in the chloroplast and in the intercellular airspace
- CABP:
-
2′ carboxyarabinitol-1,5-bisphosphate
- CA1P:
-
2′ carboxyarabinitol 1-phosphate
- gi:
-
internal conductance to CO2
- FBP:
-
fructose-1,6-bisphosphate
- GAPDH:
-
glyceraldehyde-3-phosphate dehydrogenase
- J:
-
rate of chloroplast electron transport
- k cai :
-
catalytic turnover rate
- Kc, Ko, Kr:
-
Michaelis Menten constants for CO2, O2 and RuBP of Rubisco
- K f :
-
dissociation constant of the inactive Rubisco RuBP complex
- PCO:
-
photorespiratory carbon oxidation
- PCR:
-
photosynthetic carbon reduction
- PEP:
-
phosphoenolpyruvate
- PEP case:
-
phosphoenolpyruvate carboxylase
- PGA:
-
3-phosphoglycerate
- Pi:
-
inorganic phosphate
- R d :
-
day respiration (mitochrondrial respiration other than that associated with photorespiration in the light)
- RuBP:
-
ribulose-1,5-bisphosphate
- Rubisco:
-
ribulose-1,5-bisphosphate carboxylase/oxygenase
- S c/o :
-
CO2 O2 specificity of Rubisco
- Vc, Vo:
-
rate of carboxylation and oxygenation of Rubisco
- V cmax :
-
maximal Rubisco carboxylation rate
- V omax :
-
maximal Rubisco oxygenation rate
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von Caemmerer, S., Quick, W.P. (2000). Rubisco: Physiology in Vivo. In: Leegood, R.C., Sharkey, T.D., von Caemmerer, S. (eds) Photosynthesis. Advances in Photosynthesis and Respiration, vol 9. Springer, Dordrecht. https://doi.org/10.1007/0-306-48137-5_4
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